Advertisement

Induction of apoptotic cell death by direct-current treatment in human leukemic cell lines

  • Masatsugu Kurokawa
  • Hiroshi Sakagami
  • Fumio Kokubu
  • Hiromichi Noda
  • Minoru Takeda
  • Mitsuru Adachi
Original Paper Experimental Oncology

Abstract

Exposure of human leukemic cell lines (HL-60, ML-1, U-937, MOLT-4, EOL-1) to short direct-current (d.c.) treatment induced apoptotic cell death, characterized by cell shrinkage and nuclear and internucleosomal DNA fragmentation. On the other hand, human peripheral blood lymphocytes and polymorphonuclear cells were relatively resistant to d.c treatment, and did not show any clear nuclear and DNA fragmentation. The effect of d.c. was slightly reduced by calcium depletion, but was not significantly affected by catalase or by superoxide dismutase. The present data suggest that previously reported tumor regression activities of d.c. treatment might be due, at least in part, to its apoptosis-inducing activity.

Key words

Apoptosis Direct current Antitumor activity Ca2− 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brunk CF, Jones KC, James TW (1979) Assay for nanogram quantities of DNA in cellular homogenates.Anal Biochem 92:497–500Google Scholar
  2. Chudomel V, Soucek J, Hruba A, Jerabek J, Schwarz J, Smetana K (1989) Positive effect of direct current on cytotoxicity of human lymphocytes. Neoplasma 35:573–582Google Scholar
  3. Collins SJ, Gallo RC, Gallagher RE (1977) Continuous growth and differentiation of human myeloid leukemic cells in suspension culture. Nature 270:347–349Google Scholar
  4. David SL, Absolon DR, Smith CR, Grams J, Herbert MA (1985) Effect of low level direct current on in vivo tumor growth in hamsters. Cancer Res 45:5625–5636Google Scholar
  5. Fujinaga S, Sakagami H, Kuribayashi N, Takahashi H, Amano Y, Sakagami T, Takeda M (1994) Possible role of hydrogen peroxide in apoptosis induction by ascorbic acid in human myelogenous leukemic cell lines. Showa Univ J Med Sci 6:135–144Google Scholar
  6. Gaido ML, Cidlowski JA (1991) Identification, purification, and characterization of a calcium dependent endonuclease (NUC 18) from apoptotic rat thymocytes J Biol Chem 266:18 580–18 585Google Scholar
  7. Gorczyca W, Gong J, Ardelt B, Traganos F, Darzynkiewic Z (1993) The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents. Cancer Res 53:3186–3192Google Scholar
  8. Ito H, Nakayama T, Shigematsu N, Nishiguchi I, Hashimoto S (1987) Increased anti-tumor activity of chemotherapeutic agents combined with direct current against murine transplanted tumor (in Japanese). Jpn J Cancer Chemother 14:1854–1859Google Scholar
  9. Kizaki H, Tadakuma T, Odaka C, Muramatsu J, Ishimura Y (1989) Activation of a suicide process of thymocytes through DNA fragmentation by calcium ionophores and phorbol esters. J Immunol 143:1790–1794Google Scholar
  10. Kuribayashi N, Sakagami H, Sakagami T, Niimi E, Shiokawa D, Ikekita M, Takeda M, Tanuma S (1994) Induction of DNA fragmentation in human myelogenous leukemic cell lines by sodium 5,6 benzylidene-l-ascorbate and its related compounds. Anticancer Res 14:969–976Google Scholar
  11. Kuribayashi N, Sakagami H, Iida M, Takeda M (1996) Chromatin structure and endonuclease sensitivity in human leukemic cell lines. Anticancer Res 16:1225–1230Google Scholar
  12. Mark L, James EG, George DO (1991) Effect of in vitro electrical stimulation on enhancement and suppression of malignant lymphoma cell proliferation. J Natl Cancer Inst 83:116–119Google Scholar
  13. Matsushima Y, Liu JS, Tajika E, Nagai K, Koshiishi Y, Oho K, Hayata Y (1990) Direct current therapy for local control of malignant tumors (in Japanese) Nippon-Geka-Gakkai-Zasshi 91:23–28Google Scholar
  14. McConkey DJ, Haltzell P, Nicotera P, Orrennius S (1989) Calcium-activated DNA fragmentation kills immature thymocytes FASEB J 3:1843–1849Google Scholar
  15. Minowada J, Ohnuma T, Moore GE (1972) Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived-lymphocytes. J Natl Cancer Inst 49:891–895Google Scholar
  16. Nikonova LV, Beletsky IP, Umansky SR (1993) Properties of some nuclear nucleases of rat thymocytes and their changes in radiation-induced apoptosis. Eur J Biochem 215:893–901Google Scholar
  17. Nishiguchi I (1987) The anti-tumor activity of direct current — an effective adjuvant therapy to irradiation (in Japanese). J Jpn Med Radiat 47:621–628Google Scholar
  18. Nordernstom BEW (1985) Biokenetic impacts on structure and imaging of the lung—the concept of biologically classed electric circuits; AJR 145:447–467Google Scholar
  19. Oberhammer F, Wilson JW, Dive C, Morris ID, Hickman J, Wakeling AE, Walker PR, Sikorska M (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J 12:3679–3684Google Scholar
  20. Ribeiro JM, Carson DA (1993) Ca2+/Mg2+-dependent endonuclease from human spleen: purification, properties, and role in apoptosis. Biochemistry 32:9129–9136Google Scholar
  21. Sakagami H, Hromchak R, Bloch A (1984) Prevention of phorbol ester receptor down modulation in human myelobastic leukemia ML-1 cells by differentiation-stimulating serum components. Cancer Res 44:3330–3335Google Scholar
  22. Sakagami H, Kuribayashi N, Iida M, Sakagami T, Takeda M, Fukuchi K, Gomi K, Ohata H, Momose K, Kawazoe Y, Hatano T, Yoshida T, Okuda T (1995a) Induction of DNA fragmentation by tannin and lignin-related substances. Anticancer Res 15:2121–2128Google Scholar
  23. Sakagami H, Takahashi H, Yoshida H, Yamamura M, Fukuchi K, Gomi K, Motohashi N, Takeda M (1995b) Induction of DNA fragmentation in human myelogenous leukemic cell lines by phenothiazine-related compounds. Anticancer Res 15:2533–2540Google Scholar
  24. Sakagami H, Kuribayashi N, Iida M, Hagiwara T, Takahashi H, Yoshida H, Shiota F, Ohata H, Momose K, Takeda M (1996) The requirement for and mobilization of calcium during induction by sodium ascorbate and by hydrogen peroxide of cell death. Life Sci 58:1131–1138Google Scholar
  25. Sundstrom C, Nilsson K (1976) Establishment and characterization of human histiocytic lymphoma cell line (U-937). Int J Cancer 17:565–577Google Scholar
  26. Tanuma S, Shiokawa D (1994) Multiple forms of nuclear deoxyribonuclease in rat thymocytes. Biochem Biophys Res Commun 203:789–797Google Scholar
  27. Thompson CB (1995) Apoptosis in the pathogenesis and treatment of disease. Science 267:1456–1462Google Scholar
  28. Yanagisawa-Shiota F, Sakagami H, Kuribayashi N, Iida M, Sakagami T, Takeda M (1995) Endonuclease activity and induction of DNA fragmentation in human myelogenouse leukemic cell lines. Anticancer Res 15:259–266Google Scholar
  29. Yoshie O, Majima T, Saito H (1989) Membrane oxidative metabolism of human eosinophilic cell line EOL-1 in response to phorbol diester and formyl peptide: synergistic augmentation by interferon-γ and tumor necrosis factor. J Leukoc Biol 45:10–20Google Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Masatsugu Kurokawa
    • 1
  • Hiroshi Sakagami
    • 2
  • Fumio Kokubu
    • 1
  • Hiromichi Noda
    • 1
  • Minoru Takeda
    • 2
  • Mitsuru Adachi
    • 1
  1. 1.First Department of Internal Medicine, School of MedicineShowa UniversityTokyoJapan
  2. 2.First Department of Biochemistry, School of MedicineShowa UniversityTokyoJapan

Personalised recommendations