Summary
Cell nuclei containing progesterone receptor were identified immunohistochemically in the hypothalamus and forebrain of the domestic hen using an antiserum to the steroid binding “B” subunit (110 kDa) of chicken oviduct progesterone receptor and the avidin-biotin complex procedure. Cell nuclei containing progesterone receptor were widely distributed in the anterior, medial and basal hypothalamus with the highest density occurring in the lamina terminalis and the preoptic area. Abundant, though less intensely reacting progesterone receptor was present in cell nuclei in the tuberal infundibular area and in the internal zone of the median eminence. A large group of cell nuclei containing progesterone receptor occurred in the dorsal anterior hypothalamus between the anterior commissure and the lateral ventricle. This group of nuclei extended anteriorly into the telencephalon. A small number of cell nuclei containing progesterone receptor was also found in the ventral telencephalon in the region of the nucleus accumbens.
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References
Dellmann H-D (1964) Zur Struktur des Organon vasculosum laminae terminalis des Huhnes. Anat Anz 115:174–183
Gasc JM, Ennis BW, Baulieu EE, Stumpf WE (1983) Récepteur de la progestérone dans l'oviducte de Poulet: double révélation par immunohistochimie avec des anticorps antirécepteur et par autoradiographie à l'aide d'un progestagène tritié. CR Acad Sci Paris 297 Serie 111:477–482
Gasc JM, Renoir JM, Radanyi C, Joab I, Tuohimaa P, Baulieu EE (1984) Progesterone receptor in the chick oviduct: an immunohistochemical study with antibodies to distinct receptor components. J Cell Biol 99:1193–1201
Hsu SM, Rain EL, Fanger H (1981) The use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Ibata Y, Watanabe K, Kinoshita H, Kubo S, Sano Y, Sin S, Hashimura E, Imagawa K (1979) Detection of catecholamine and luteinizing hormone-releasing hormone (LH-RH) containing nerve endings in the median eminence and the organum vasculosum laminae terminalis by fluorescence histochemistry and immunohistochemistry on the same microscopic sections. Neurosci Lett 11:181–186
Kawashima M, Kamiyoshi M, Tanaka K (1978) A cytoplasmic progesterone receptor in hen pituitary and hypothalamic tissues. Endocrinology 102:1207–1213
Kawashima M, Kamiyoshi M, Tanaka K (1979a) Cytoplasmic progesterone receptor concentrations in the hen hypothalamus and pituitary: Difference between laying and non-laying hens and changes during the ovulatory cycle. Biol Reprod 20:581–585
Kawashima M, Kamiyoshi M, Tanaka K (1979b) Nuclear progesterone receptor in the hen pituitary and hypothalamus. Endocrinol Jpn 26:501–506
Martinez-Vargas MC, Stumpf WE, Sar M (1976) Anatomical distribution of estrogen target cells in the avian CNS: A comparison with the mammalian CNS. J Comp Neurol 167:83–104
Mikami S (1976) Ultrastructure of the organum vasculosum of the lamina terminalis of the Japanese quailCoturnix coturnix japonica. Cell Tissue Res 172:227–243
Moore RY (1977) Organum vasculosum lamina terminalis: Innervation by serotonin neurons of the midbrain raphe. Neurosci Lett 5:297–302
Ralph CL, Fraps RM (1960) Induction of ovulation in the hen by injection of progesterone into the brain. Endocrinology 66:269–272
Renoir JM, Mester J, Buchou T, Catelli MG, Tuohimaa P, Binart N, Joab I, Radanyi C, Baulieu EE (1984) Purification by affinity chromatography and immunological characterization of 110 kDa component of the chick oviduct progesterone receptor. Biochem J 218:685–692
Rhees RW, Abel JH, Haack DW (1972) Uptake of tritiated steroids in the brain of the duck (Anas platyrhynchos). An autoradiographic study. Gen Comp Endocrinol 18:292–300
Sharp PJ (1980) In: Epple A, Stetson MH (eds) Avian endocrinology. Academic Press, London New York, pp 435–454
Sterling RJ, Sharp PJ (1982) The localisation of LH-RH neurones in the diencephalon of the domestic hen. Cell Tissue Res 222:283–298
Sterling RJ, Gasc JM, Sharp PJ, Tuohimaa P, Baulieu EE (1984) Absence of nuclear progesterone receptor in LH releasing hormone neurones in laying hens. J Endocrinol 102:R5-R7
Stumpf WE, Gasc JM, Baulieu EE (1983) Progesterone receptor in pituitary and brain: combined autoradiography-immunohistochemistry with tritium-labelled ligand and receptor antibodies. Mikroskopie 40:359–363
Tienhoven A van, Juhasz LP (1962) The chicken telencephalon, diencephalon, and mesencephalon in stereotaxic co-ordinates. J Comp Neurol 118:185–197
Tuohimaa P, Renoir JM, Radanyi C, Mester J, Joab I, Buchou T, Baulieu EE (1984) Antibodies against highly purified B-subunit of the chick oviduct progesterone receptor. Biochem Biophys Res Commun 119:433–439
Wood-Gush DGM, Gilbert AB (1973) Some hormones involved in the nesting behaviour of hens. Anim Behav 21:98–103
Wood-Gush DGM, Langley GAS, Leitch AF, Gentle MJ, Gilbert AB (1977) An autoradiographic study of sex steroids in the chicken telencephalon. Gen Comp Endocrinol 31:161–168
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Sterling, R.J., Gasc, J.M., Sharp, P.J. et al. The distribution of nuclear progesterone receptor in the hypothalamus and forebrain of the domestic hen. Cell Tissue Res. 248, 201–205 (1987). https://doi.org/10.1007/BF01239981
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DOI: https://doi.org/10.1007/BF01239981