Summary
Adult mice were found to show regional variation in the epithelial expression of some molecules of the blood-group antigen series. To investigate connective tissue influences on such differences, heterotypic recombinants of epithelia and connective tissues from various regions were prepared and examined using monoclonal antibodies directed against bloodgroup antigens H and Ley. The results indicate that epithelia may maintain a preexisting regionally specific pattern following recombination but that, in some recombinant matches, the connective tissue is capable of signalling redirection of the pattern of expression towards that typical of the epithelium with which it is normally associated.
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References
Abe K, McKibbin JM, Hakomori S (1983) The monoclonal antibody directed to difucosylated type 2 chain (Y Determinant). J Biol Chem 258:11793–11797
Cunha GR, Fujii H, Neubauer BL, Shannon JM, Sayer L, Reese RA (1983) Epithelial-mesenchymal interactions in prostatic development. I. Morphological observations of prostatic induction by urogenital sinus mesenchyme in epithelium of the adult rodent urinary bladder. J Cell Biol 96:1662–1670
Dabelsteen E, Vedtofte P, Hakomori S, Young WW (1982) Carbohydrate chains specific for blood group antigens in differentiation of human oral epithelium. J Invest Dermatol 79:3–7
Dabelsteen E, Buschard K, Hakomori S, Young WW (1984) Pattern of distribution of blood group antigen on human epidermal cells during maturation. J Invest Dermatol 82:13–17
Edelman GM (1983) Cell adhesion molecules. Science 219:450–457
Edelman GM, Gallin WJ, Delouvee A, Cunningham BA, Thiery J-P (1983) Early epochal maps of two different cell adhesion molecules. Proc Natl Acad Sci USA 80:4384–4388
Feizi T (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens. Nature 314:53–58
Flcischmajcr R, Billingham RE (1968) Epithelial-Mesenchymal Interactions. Williams and Wilkins, Baltimore
Fukushi Y, Hakomori S, Nudelman E, Cochran N (1984) Novel fucolipids accumulating in human adenocarcinoma. II. Selective isolation of hybridoma antibodies that differentially recognize mono-, di-, and trifucosylated type 2 chain. J Biol Chem 259:4681–4685
Gooi HC, Feizi T, Kapadia A, Knowles BB, Solter D, Evans MJ (1981) Stagespecific embryonic antigen involves alpha 1–3 fucosylated type 2 blood group chains. Nature 292:156–158
Hakomori S (1981a) Glycosphingolipids in cellular interactions: differentiation and oncogenesis. Ann Rev Biochem 50:733–764
Hakomori S (1981b) Blood group A, B, H and Ii antigens of human erythrocytes: chemistry, polymorphism, and their developmental changes. Semin Hematol 18:39–62
Hill MW, Mackenzie IC (1984) The influence of differing connective tissue substrates on the maintenance of adult stratified squamous epithelia. Cell Tissue Res 237:473–478
Holmes EH, Ostrander GK, Hakomori S (1985) Enzymatic basis for the accumaulation of glycolipids with X and dimeric X determinants in human lung cancer cells. J Biol Chem 260:7619–7627
Johnson GD, Nogueira Araujo GMC (1981) A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Methods 43:349–350
Mackenzie IC, Hill MW (1981) Maintenance of regionally-specific patterns of cell proliferation and differentiation in transplanted skin and oral mucosa. Cell Tissue Res 219:597–607
Mackenzie IC, Hill MW (1984) Connective tissue influences on patterns of epithelial architecture and keratinization in skin and oral mucosa of the adult mouse. Cell Tissue Res 235:551–559
Reibel J, Dabelsteen E, Hakomori S, Young WW, Mackenzie IC (1984) The distribution of blood group antigens in rodent epithelia. Cell Tissue Res 237:111–116
Sawyer RH, Fallon JF eds (1983) Epithelial-Mesenchymal Interactions in Development. Praeger Publishers, New York
Schweizer J, Winter H, Hill MW, Mackenzie IC (1984) The keratin polypeptide patterns in heterotypically recombined epithelia of skin and mucosa of adult mouse. Differentiation 26:144–153
Sengel P (1976) Morphogenesis of Skin. Cambridge University Press, London
Watanabe K, Hakomori S (1976) Status of blood group carbohydrate chains in ontogenesis and in oncogenesis. J Exp Med 144:644–653
Young WW, Pofgoukalian J, Hakomori S (1981) Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-N-glycosyl type H chain. J Biol Chem 256:10967–10972
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This work was supported by NIH-NIDR RO1-DEO-5190
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Mackenzie, I.C., Dabelsteen, E. Connective tissue influences on the expression of epithelial cell-surface antigens. Cell Tissue Res. 248, 137–141 (1987). https://doi.org/10.1007/BF01239974
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DOI: https://doi.org/10.1007/BF01239974