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Neuroregulation of protein synthesis in odontoblasts of the first molar of the rat after wounding

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Summary

Odontoblasts respond to occlusal trauma by increased elaboration of a matrix which is subsequently calcified to form reparative dentin. The purpose of the present study was to analyze quantitatively and compare the ability of odontoblasts to synthesize collagen after wounding in rats with an intact innervation (baseline) and in rats with sensory (inferior alveolar nerve, IAN) and/or sympathetic (superior cervical ganglion, SCG) surgical denervation. Surgery was performed 7 days prior to wounding. All rats had 1 mm of enamel and dentin removed from the occlusal surface of the first mandibular molar (resected side) with the contralateral tooth serving as a control. Rats were killed 1 h after injection with3H-proline on days 0, 5, 10 or 15 after wounding, and mandibles were removed and processed for autoradiography. Grain counts were performed over odontoblasts throughout the pulp horns for each time period and for control and experimental molars in intact (baseline) and denervated groups. When compared to the control baseline, the experimental baseline data showed increased3-proline uptake throughout the study with a peak at 5 days. When compared to the baseline data, IAN and SCG results demonstrated a delay or attenuation of the protein synthetic response. The results indicate that the sensory and sympathetic neural components may regulate odontoblastic response to wounding.

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Authors and Affiliations

  1. Department of Oral Biology, University of Michigan, School of Dentistry, Ann Arbor, Michigan, USA

    Daniel J. Chiego Jr. & James K. Avery

  2. Department of Anatomy/Division of Cell Biology, University of Kansas, College of Health Sciences and Hospital, Kansas City, Kansas, USA

    Robert M. Klein

Authors
  1. Daniel J. Chiego Jr.
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  2. James K. Avery
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  3. Robert M. Klein
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Supported by a grant from the National Institutes of Health (DE05636)

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Chiego, D.J., Avery, J.K. & Klein, R.M. Neuroregulation of protein synthesis in odontoblasts of the first molar of the rat after wounding. Cell Tissue Res. 248, 119–123 (1987). https://doi.org/10.1007/BF01239971

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  • DOI: https://doi.org/10.1007/BF01239971

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