Abstract
Cations such as Cu2+, Cr3+ and Hg+ can be separated on silica gel thin layer Chromatographic plates. The developing solution contains 0.02M EDTA at pH 2.5. For visualization, the plate was pretreated with ethidium bromide, a strong fluorescer. Quenching of the red fluorescence can be observed visually even down to the 0.1 nmole range per spot.
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Ma, Y.F., Yeung, E.S. Detection of cations separated by thin-layer chromatography by fluorescence quenching of ethidium bromide. Mikrochim Acta 96, 327–332 (1988). https://doi.org/10.1007/BF01236117
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DOI: https://doi.org/10.1007/BF01236117