Abstract
Instrumentation of a high-resolution optical scanning fluorescence microscope is presented. This instrument provides a fluorescent image of the structural surface of various kinds of thin membrane samples with high resolution on the molecular scale. For this instrument, a new excitation optical tip and a three-dimensional scanning unit have been designed. The optical tip consists of a sharpened optical fibre covered with evaporated aluminium in vacuo, on which the top has a pinhole. The optical tip is mounted in the centre of a crosslinked piezoelectric scanning head. On thez-axis, a piezoelectric positioner is equipped with a differential micrometer mechanism. All operations are controlled with a micro-computer system.
The scanning area in this instrument is 4000*4000 nm2 for measurements of biological specimens. Several optical scanning microscopic images as well as the STM image are presented.
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Okazaki, S., Sasatani, H., Hatano, H. et al. Development of high-resolution optical scanning fluorescence microscopy. Mikrochim Acta 96, 87–95 (1988). https://doi.org/10.1007/BF01236094
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DOI: https://doi.org/10.1007/BF01236094