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Sub-femtomole determination of DABSYL-amino acids with capillary zone electrophoresis separation and laser-induced thermo-optical absorbance detection

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Abstract

Fifteen amino acids are determined as the DABSYL (4-4-dimethylaminoazobenzene-4'-sulfonyl chloride) derivative with capillary zone electrophoresis separation and low-power laser-induced thermo-optical detection. Separation efficiencies are on the order of 200 000 theoretical plates and the detection limit, 3 s, is 200 attomole of glycine injected onto the column. At the detection limit, 0.7 attomole of glycine is present within the 40 picoliter detection volume.

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Authors and Affiliations

  1. Department of Chemistry, University of Alberta, T6G 2G2, Edmonton, Alberta, Canada

    Margaret Yu & Norman J. Dovichi

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  1. Margaret Yu
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  2. Norman J. Dovichi
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Yu, M., Dovichi, N.J. Sub-femtomole determination of DABSYL-amino acids with capillary zone electrophoresis separation and laser-induced thermo-optical absorbance detection. Mikrochim Acta 96, 27–40 (1988). https://doi.org/10.1007/BF01236090

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  • DOI: https://doi.org/10.1007/BF01236090

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