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Physical mapping of chromosome 21 DNA markers in Alzheimer's disease region using somatic cell hybrids

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Somatic Cell and Molecular Genetics

Abstract

From a chromosome 21 phage library, we selected 10 clones located proximal of the senile plaque amyloid precursor protein gene. Since a locus for Alzheimer's disease (AD) has been localized in the pericentromeric region of chromosome 21, the selected phage clones are potential candidate probes for genetic analysis of AD. In this study, we subcloned single-copy fragments of the selected phage clones, refined their physical localization, and examined their chromosomal distribution in relation to their position on chromosome 21. The results indicated that the phage clones are identifying nine chromosome 21 loci, which, if polymorphic, may be helpful in localizing the AD locus more precisely. Moreover, since all phage clones are located close to the centromere of chromosome 21, they can be used to determine the parental origin of nondisjunction in trisomy 21 with high reliability.

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Authors and Affiliations

  1. Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium

    Guy Van Camp, Wim Van Hul, Hubert Backhovens, Piet Stinissen, Anita Wehnert, Antoon Vandenberghe & Christine Van Broeckhoven

  2. Innogenetics, Industriepark Zwijnaarde, B-9710, Gent, Belgium

    Hubert Backhovens & Anita Wehnert

  3. Eleanor Roosevelt Institute, 1899 Gaylord Street, 80206, Denver, Colorado

    David Patterson

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  1. Guy Van Camp
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Van Camp, G., Van Hul, W., Backhovens, H. et al. Physical mapping of chromosome 21 DNA markers in Alzheimer's disease region using somatic cell hybrids. Somat Cell Mol Genet 16, 241–249 (1990). https://doi.org/10.1007/BF01233360

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  • DOI: https://doi.org/10.1007/BF01233360

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