Abstract
Syndecan, a cell surface proteoglycan, is an integral membrane protein acting as a receptor for the extracellular matrix. For chromosomal localization of the human syndecan gene, a panel of mouse-human somatic cell hybrids was analyzed by Southern blotting using the cDNA probe for human syndecan. The hybrids were karyotyped at the time of DNA extraction. A band corresponding to the human syndecan gene in Southern blots was found only in a hybrid cell line containing human chromosome 2. This hybrid was subcloned and its subclones were analyzed by Southern blotting and karyotyped. Subclones carrying human chromosome 2 contained the syndecan gene, while subclones not carrying this chromosome did not. The human syndecan gene is thus assigned to chromosome 2.
Literature cited
Rapraeger, A.C., and Bernfield, M. (1983).J. Biol. Chem. 2583632–3636.
Rapraeger, A., and Bernfield, M. (1985).J. Biol. Chem. 2604103–4109.
Jalkanen, M., Nguyen, H., Rapraeger, A., Kurn, N., and Bernfield, M. (1985).J. Cell Biol. 101976–984.
Rapraeger, A., Jalkanen, M., Endo, E., Koda, J.E., and Bernfield, M. (1985).J. Biol. Chem. 26011046–11052.
Saunders, S., Jalkanen, M., O'Farrell, S., and Bernfield, M. (1989).J. Cell Biol. 1081547–1556.
Koda, J.E., Rapraeger, A., and Bernfield, M. (1985).J. Biol. Chem. 2608157–8162.
Saunders, S., and Bernfield, M. (1988).J. Cell Biol. 106423–430.
Sun, X., Mosher, D.F., and Rapraeger, A. (1989).J. Biol. Chem. 2642885–2889.
Sanderson, R.D., and Bernfield, M. (1988).Proc. Natl. Acad. Sci. U.S.A. 859562–9566.
Rapraeger, A., Jalkanen, M., and Bernfield, M. (1986).J. Cell Biol. 1032683–2696.
Hayashi, K., Hayashi, M., Jalkanen, M., Firestone, J.H., Trelstad, R.L., and Bernfield, M. (1987).J. Histochem. Cytochem. 351079–1088.
Bernfield, M., Rapraeger, A., Jalkanen, M., and Banerjee, S.D. (1985). InBasement Membranes (ed.) Shibata, S. (Elsevier Science Publishing, New York), pp. 343–352.
Thesleff, I., Jalkanen, M., Vainio, S., and Bernfield, M. (1988).Dev. Biol. 129565–572.
Vainio, S., Jalkanen, M., and Thesleff, I. (1989).J. Cell Biol. 1081945–1954.
Vainio, S., Lehtonen, E., Jalkanen, M., Bernfield, M., and Saxen, L. (1989).Dev. Biol. 134382–391.
Sanderson, R.D., Lalor, P., and Bernfield, M. (1989).Cell Reg. 127–35.
Mali, M., Jaakkola, P., Arvilommi, A.-M., and Jalkanen, M. (1990).J. Biol. Chem. 2656884–6889.
Suomalainen, H.A., Goldsby, R.A., Osborne, B.A., and Schröder, J. (1980).Scand. J. Immunol. 11163–168.
Maniatis, T., Fritsch, E.F., and Sambrook, J. (1990).Molecular Cloning, A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York), pp. 280–281.
Seabright, M.A. (1971).Lancet 2971–972.
Southern, E.M. (1975).J. Mol. Biol. 98503–517.
Feinberg, A.P., and Vogelstein, B. (1984).Anal. Biochem. 1326–13.
Koller, B.H., Sidwell, B., DeMars, R., and Orr, H.T. (1984).Proc. Natl. Acad. Sci. U.S.A. 815175–5178.
Croce, C.M., Shander, M., Martinis, J., Cicurel, L., D'Ancona, G.G., Dolby, T.W., and Koprowski, H. (1979).Proc. Natl. Acad. Sci. U.S.A. 763416–3419.
Hobart, M.J., Rabbits, T.H., Goodfellow, P.N., Solomon, E., Chambers, S., Spurr, N., and Povey, S. (1981).Ann. Hum. Genet. 45331–335.
Flanagan, J.G., and Rabbits, T.H. (1982).Nature 300709–713.
Johnson, M.J., Natali, A.M., Cann, H.M., Honjo, T., and Cavalli-Sforza, L.L. (1984).Proc. Natl. Acad. Sci. U.S.A. 817840–7844.
Junien, C., and McBride, O.W. (1989).Cytogenet. Cell Genet. 51226–258.
van den Elsen, P., Bruns, G., Gerhard, D.S., Pravtcheva, D., Jones, C., Housman, D., Ruddle, F.A., Orkin, S., and Terhorst, C. (1985).Proc. Natl. Acad. Sci. U.S.A. 822920–2924.
Gold, D.P., van Dongen, J.J.M., Morton, C.C., Bruns, G.A.P., van den Elsen, P., van Kessel, Ad H.M.G., and Terhorst, C. (1987).Proc. Natl. Acad. Sci. U.S.A. 841664–1668.
Krissansen, G.W., Gorman, P.A., Kozak, C.A., Spurr, N.K., Sheer, D., Goodfellow, P.N., Crumpton, M.J. (1987).Immunogenetics 26258–266.
Forsberg, U.H., Ala-Kapee, M.M., Jalkanen, S., Andersson, L.C., and Schröder, J. (1989).Eur. J. Immunol. 19409–412.
Forsberg, U.H., Jalkanen, S., and Schröder, J. (1989).Immunogenetics 29405–407.
Forsberg, U.H., Bazil, V., Stefanova, I., and Schröder, J. (1989).Immunogenetics 30188–193.
Rettig, W.J., Real, F.X., Spengler, B.A., Biedler, J.L., and Old, L.J. (1986).Science 2311281–1284.
Marynen, P., Zhang, J., Cassiman, J.-J., Van den Berghe, H., and David, G. (1989).J. Biol. Chem. 2647017–7024.
Hynes, R.O., Marcantonio, E.E., Stepp, M.A., Urry, L.A., and Yee, G.H.J. Cell. Biol. 109:409–420.
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Ala-Kapee, M., Nevanlinna, H., Mali, M. et al. Localization of gene for human syndecan, an integral membrane proteoglycan and a matrix receptor, to chromosome 2. Somat Cell Mol Genet 16, 501–505 (1990). https://doi.org/10.1007/BF01233200
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DOI: https://doi.org/10.1007/BF01233200