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Physical mapping of loci in the distal half of the short arm of the human X chromosome: Implications for the spreading of X-chromosome inactivation

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Somatic Cell and Molecular Genetics

Abstract

The relative order of 11 loci in the distal half of the short arm of the human X chromosome was examined using a panel of somatic cell hybrids containing structurally rearranged X chromosomes. The results show that the gene for phosphoribosylpyrophosphate synthetase 2 (PRPS2) is located betweenZFX (zinc finger protein, X-linked) andSTS (steroid sulfatase). The results also confirm the localization ofZFX distal toPOLA (α-DNA polymerase). Previous studies have shown thatSTS andZFX escape X-inactivation whereasPOLA undergoes inactivation. Evaluation ofPRPS2 expression in somatic cell hybrids containing inactive human X chromosomes showed thatPRPS2 undergoes X-inactivation. These results provide further evidence for interspersion of loci that do and do not undergo X-inactivation on the human X chromosome.

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Author notes

  1. J. C. Wang & J. Ellison

    Present address: Prenatal Diagnostic Center, 02173, Lexington, Massachusetts

  2. L. J. Shapiro

    Present address: Department of Pediatrics, University of California, 94143, San Francisco, California

Authors and Affiliations

  1. Division of Medical Genetics, Harbor-UCLA Medical Center, 90509, Torrance, California

    J. C. Wang, M. B. Passage, J. Ellison, P. H. Yen, L. J. Shapiro & T. K. Mohandas

  2. Department of Medicine, University of Chicago, 60637, Chicago, Illinois

    M. A. Becker

Authors
  1. J. C. Wang
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  2. M. B. Passage
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  3. J. Ellison
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  4. M. A. Becker
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  5. P. H. Yen
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  6. L. J. Shapiro
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  7. T. K. Mohandas
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Wang, J.C., Passage, M.B., Ellison, J. et al. Physical mapping of loci in the distal half of the short arm of the human X chromosome: Implications for the spreading of X-chromosome inactivation. Somat Cell Mol Genet 18, 195–200 (1992). https://doi.org/10.1007/BF01233165

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  • DOI: https://doi.org/10.1007/BF01233165

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