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Somatic Cell and Molecular Genetics

, Volume 18, Issue 2, pp 103–111 | Cite as

Ablation of stimulation of a cAMP-responsive promoter in CHO cell lines defective in their cAMP-dependent protein kinase system

  • Robert D. Fleischmann
  • Clifford Jeng
  • Michael M. Gottesman
Article

Abstract

We have studied the requirement for an intact cAMP-dependent protein kinase (PKA) system to regulate cAMP-mediated gene transcription in Chinese hamster ovary (CHO) cells. Wild-type CHO cells and mutant CHO cell lines selected for their resistance to the growth inhibitory effect of 8-Br-cAMP and defective in their PKA system were transiently transfected with reporter plasmids containing 2.5 and 3.0 kb of the 5′-flanking sequence of the rat tyrosine aminotransferase (TAT) gene promoter. This segment of DNA contains no CRE-like sequences, yet wild-type transfectants exhibited a specific increase in TAT promoter activity following growth in medium containing 8-Br-cAMP. In CHO cell lines defective in their PKA, the transfected TAT promoter failed to respond to cAMP treatment. We conclude that an intact PKA system is necessary for the cAMP-mediated increase in TAT promoter activity in CHO cells and that there is no requirement for a CRE to see this effect.

Keywords

Chinese Hamster Ovary Cell Chinese Hamster Ovary Reporter Plasmid Growth Inhibitory Effect Specific Increase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Robert D. Fleischmann
    • 1
  • Clifford Jeng
    • 1
  • Michael M. Gottesman
    • 1
  1. 1.Laboratory of Cell Biology, National Cancer InstituteNational Institutes of HealthBethesda

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