Somatic Cell and Molecular Genetics

, Volume 18, Issue 2, pp 103–111 | Cite as

Ablation of stimulation of a cAMP-responsive promoter in CHO cell lines defective in their cAMP-dependent protein kinase system

  • Robert D. Fleischmann
  • Clifford Jeng
  • Michael M. Gottesman


We have studied the requirement for an intact cAMP-dependent protein kinase (PKA) system to regulate cAMP-mediated gene transcription in Chinese hamster ovary (CHO) cells. Wild-type CHO cells and mutant CHO cell lines selected for their resistance to the growth inhibitory effect of 8-Br-cAMP and defective in their PKA system were transiently transfected with reporter plasmids containing 2.5 and 3.0 kb of the 5′-flanking sequence of the rat tyrosine aminotransferase (TAT) gene promoter. This segment of DNA contains no CRE-like sequences, yet wild-type transfectants exhibited a specific increase in TAT promoter activity following growth in medium containing 8-Br-cAMP. In CHO cell lines defective in their PKA, the transfected TAT promoter failed to respond to cAMP treatment. We conclude that an intact PKA system is necessary for the cAMP-mediated increase in TAT promoter activity in CHO cells and that there is no requirement for a CRE to see this effect.


Chinese Hamster Ovary Cell Chinese Hamster Ovary Reporter Plasmid Growth Inhibitory Effect Specific Increase 
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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Robert D. Fleischmann
    • 1
  • Clifford Jeng
    • 1
  • Michael M. Gottesman
    • 1
  1. 1.Laboratory of Cell Biology, National Cancer InstituteNational Institutes of HealthBethesda

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