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The Chinese hamster V79 cell mutant V-H4 is phenotypically like Fanconi anemia cells

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Somatic Cell and Molecular Genetics

Abstract

It has been shown by genetic complementation analysis that a mitomycin C-sensitive mutant (V-H4) of Chinese hamster V79 cells is the first rodent equivalent of Fanconi anemia (FA) group A. The V-H4 mutant shows many typical characteristics of cells derived from FA patients. V-H4 cells exhibit increased sensitivity towards cross-linking agents as MMC (∼30-fold), cis-DDP (∼10-fold), DEB (∼10-fold), and PUVA (∼1.6-fold), but an only slightly increased sensitivity to monofunctional alkylating agents (EMS and MMS) and actinomycin D. V-H4 cells are also moderately sensitive to adriamycin (1.6-fold), and not sensitive to H2O2. The levels of chromosomal aberrations induced by MMC and cis-DDP treatment are higher (4- to 6-fold) in V-H4 cells than in the wild-type V79 cells. Genetic complementation analysis with other Chinese hamster mutants hypersensitive to MMC (irs1, irs1SF, UV20 and UV41) indicates clearly that V-H4 belongs to a different, new complementation group. This unique mutant is very stable and can serve as a vehicle to isolate the complementing FA-A gene from normal human DNA.

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Authors and Affiliations

  1. MGC, Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands

    M. Z. Zdzienicka, I. Neuteboom & J. W. I. M. Simons

  2. J.A. Cohen Institute, Interuniversity Research Institute for Radiopathology and Radiation Protection, Leiden

    M. Z. Zdzienicka

  3. Department of Anthropogenetics, Free University, Amsterdam, The Netherlands

    F. Arwert & M. Rooimans

Authors
  1. M. Z. Zdzienicka
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  2. F. Arwert
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  3. I. Neuteboom
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  4. M. Rooimans
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  5. J. W. I. M. Simons
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Zdzienicka, M.Z., Arwert, F., Neuteboom, I. et al. The Chinese hamster V79 cell mutant V-H4 is phenotypically like Fanconi anemia cells. Somat Cell Mol Genet 16, 575–581 (1990). https://doi.org/10.1007/BF01233098

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  • DOI: https://doi.org/10.1007/BF01233098

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