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The use of human repetitive DNA to target selectable markers into only the human chromosome of a human-hamster hybrid cell line (AL)

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Somatic Cell and Molecular Genetics

Abstract

We used the plasmid BLUR-8 that contains an 800-base pair (bp) sequence of human repetitiveAlu DNA in a cotransfection protocol to target the plasmids pSV2neo or EBO-pcD-leu-2 (hygro) into a single site of the sole human chromosome, number 11, of a Chinese hamster-human hybrid cell line (AL). The neo and hygro plasmids confer resistance to antibiotics G418 and hygromycin, respectively. Of the 33 cotransfected clones with single-site insertions, 1/13 without BLUR-8 and 6/20 with BLUR-8 were only in human chromosome 11. A frequency of insertion of 1/13 is not different than expected by chance (ϱ=0.3512). On the other hand, the probability that 6/20 insertions, as seen with BLUR-8, occurred by chance is low (ϱ=0.0003). We suggest that the human DNA sequences contained in BLUR-8 targeted insertions into only the human chromosome.

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Authors and Affiliations

  1. Radiological Health Sciences, Colorado State University, 80523, Fort Collins, Colorado

    Charles Waldren, Mike Braaton, Diane Vannais & Bijan Fouladi

  2. The Johns Hopkins Medical Institutions, 21205, Baltimore, Maryland

    R. D. Parker

Authors
  1. Charles Waldren
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  2. Mike Braaton
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  3. Diane Vannais
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  4. Bijan Fouladi
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  5. R. D. Parker
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Waldren, C., Braaton, M., Vannais, D. et al. The use of human repetitive DNA to target selectable markers into only the human chromosome of a human-hamster hybrid cell line (AL). Somat Cell Mol Genet 18, 417–422 (1992). https://doi.org/10.1007/BF01233081

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  • DOI: https://doi.org/10.1007/BF01233081

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