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Evaluation of the mutagenic effects of SV40 in mouse, hamster, and mouse-human hybrid cells

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Somatic Cell and Molecular Genetics

Abstract

We have examined the ability of SV40 to induce changes in drug or temperature resistance in mouse, hamster, and mouse-human hybrid cells. SV40 induced a substantial increase of cells resistant to 5-bromodeoxyuridine + trifluorothymidine in Balb/c 3T3 cells and induced an increase of hybrid cells resistant to 6-thioguanine. SV40 was found to be nonmutagenic or weakly mutagenic in other test systems. The 3T3 cells were T-antigen positive, exhibited a marked reduction in TK activity, were heterogeneous for [3H]BrdU incorporation by autoradiography, and exhibited instability of the drug-resistance phenotype, suggesting that SV40 may be inducing resistance by an epigenetic process. SV40-induced 6-thioguanine resistance in the hybrids appears to occur predominantly by chromosome loss.

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Author notes

  1. Richard E. Giles

    Present address: Department of Veterinary Microbiology and Pathology, Washington State University, 99164-7040, Pullman, Washington

  2. Frederick M. Boyce

    Present address: Department of Genetics, Enders 560, Children's Hospital, 300 Longwood Ave., 02115, Boston, Massachusetts

Authors and Affiliations

  1. Department of Microbiology, The University of Michigan Medical School, 48109, Ann Arbor, Michigan

    Richard E. Giles, Frederick M. Boyce & William W. Brockman

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  1. Richard E. Giles
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  2. Frederick M. Boyce
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  3. William W. Brockman
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Giles, R.E., Boyce, F.M. & Brockman, W.W. Evaluation of the mutagenic effects of SV40 in mouse, hamster, and mouse-human hybrid cells. Somat Cell Mol Genet 17, 327–339 (1991). https://doi.org/10.1007/BF01233058

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  • DOI: https://doi.org/10.1007/BF01233058

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