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Instability of immunoglobulin genes in S107 cell line

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Somatic Cell and Molecular Genetics

Abstract

Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.

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Authors and Affiliations

  1. Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461, Bronx, New York

    Seung-Uon Shin, Ronald DePinho, Donald J. Zack & Matthew D. Scharff

  2. National Cancer Institute, Bethesda, Maryland

    Stuart Rudikoff

Authors
  1. Seung-Uon Shin
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  2. Ronald DePinho
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  3. Donald J. Zack
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  4. Stuart Rudikoff
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  5. Matthew D. Scharff
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Shin, SU., DePinho, R., Zack, D.J. et al. Instability of immunoglobulin genes in S107 cell line. Somat Cell Mol Genet 17, 259–276 (1991). https://doi.org/10.1007/BF01232821

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  • DOI: https://doi.org/10.1007/BF01232821

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