Summary
By the method ofKoelle andFriedenwald a high cholinesterase activity has been established in the encapsulated receptors in the mucosa of the cat's tongue. Following brief incubation with acetyl substrate a positive reaction developed in the area surrounding the axon, named inner bulb for all receptors of this type. The point of contact between the axon and the inner bulb shows the highest cholinesterase activity. Following incubation with butyryl substrate a strongly positive reaction developed in the inner bulb only in some receptors situated in the depth of the submucosa.
By the combined method of cholinesterase demonstration according toKoelle andFriedenwald with subsequent impregnation according toGros it has been shown, that the submucosal areas, stained by the first method really represent components of these receptors. The area in the center of the bulb, which remains “empty” following the histochemical reaction is occupied by an axon running through it and demonstrated by the second method. The site of contact between the axon and the inner bulb, which according to the first method, is more strongly positive than the rest of the bulb, becomes even more intensively stained according to the second method. The elements of the fibrous capsule are demonstrated only by the second method.
It is assumed that the high cholinesterase activity of the inner bulb is not peculiar for these receptors alone.
Zusammenfassung
Mittels der Koelle- und Friedenwald-Methode wurde eine hohe Cholinesteraseaktivität in den eingekapselten Rezeptoren der Katzenzungenschleimhaut gefunden. Nach kurzer Inkubation mit Acetylsubstrat entwickelte sich eine positive Reaktion in dem Bereich rund um das Axon (genannt innerer Kolben aller Rezeptoren dieses Typus). Am Berührungspunkt des Axons und des inneren Kolbens ist die Aktivität der Cholinesterase am höchsten. Nach einer Inkubation mit Butyrylsubstrat entwickelte sich eine stark positive Reaktion in nur einigen in der Tiefe der Submucosa gelegenen Rezeptoren.
Durch die kombinierte Cholinesterasebestimmung nachKoelle undFriedenwald mit darauffolgender Impregnation nachGros wurde festgestellt, daß jene Stellen der Submucosa, die nach der ersten Methode gefärbt wurden, tatsächlich Bestandteile dieser Rezeptoren darstellen. Der mittlere Abschnitt des Kolbens, der nach der histochemischen Reaktion leer bleibt, wird von einem hier durchlaufenden Axon eingenommen, das erst nach der zweiten Methode zur Darstellung kommt. Die Berührungstellee zwischen dem Axon und dem Kolben, die nach der ersten Methode stärker positiv als der Rest des Kolbens ist, wird nach der zweiten Methode noch intensiver. Die Elemente der fibrösen Kapsel werden erst nach Anwendung der zweiten Methode sichtbar.
Man nimmt an, daß die hohe Aktivität der Cholinesterase der inneren Kolben keine spezifische Eigenschaft dieser Rezeptoren darstellt.
Résumé
Par la méthode de Koelle et Friedenwald, une haute activité cholinestérasique a été observée dans les récepteurs encapsulés de la muqueuse de la langue du chat. Après une brève incubation avec un substrat d'acétyle, une réaction positive se développa dans la région entourant l'axone, appelée bulbe intérieur. Au point de contact entre l'axone et le bulbe intérieur, l'activité de la cholinestérase est maximale. Après une incubation avec un substrat de butyryle, une réaction fortement positive se développa dans les bulbes intérieurs, mais seulement dans quelques récepteurs situés dans les profondeur de la submuqueuse.
Par une méthode combinée démontrant l'activité de la cholinestérase d'aprèsKoelle etFriedenwald, wuivie de l'imprégnation d'aprèsGros, il a été prouvé que les régions colorées selon la première méthode représentent réellement des parties de ces récepteurs. La région au centre du bulbe restée vide après la réaction histochimique est occupée par un axone qui la traverse, ce qui est démontré par la seconde méthode. Le lieu de contact entre l'axone et le bulbe intérieur étant plus fortement positif que le reste du bulbe d'après la première méthode, devient, d'après la seconde méthode, même encore plus intense. Les éléments de la capsule fibreuse ne deviennent visibles que par la seconde méthode.
On présume que la haute activité de la cholinestérase du bulbe intérieur n'est pas une particularité de ces récepteurs seulement.
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Spassova, I. On the cholinesterase activity of encapsulated nerve endings in the tongue of the cat. Acta Neurovegetativa 29, 529–551 (1967). https://doi.org/10.1007/BF01231306
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DOI: https://doi.org/10.1007/BF01231306