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Co-expression of CD4 and CD8 associated with elevated interleukin-4 in a cord T cell line derived by cocultivating normal cord leukocytes and an HTLV-II-producing simian leukocyte cell line (Si-IIA)

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  • Experimental Oncology
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Summary

A new interleukin-2(IL-2)-dependent T cell line, designated CS-IIA, was established by cocultivating normal human cord leukocytes and a lethally X-irradiated HTLV-II-producing simian leukocyte cell line (Si-IIA). CS-IIA showed CD4 dominance during the early culture. However, after addition of IL-2, CS-IIA predominantly co-expressed CD4 and CD8 (69.5%) and also expressed the surface markers CD1−, CD3+, CD19−, CD25+ and HLA-DR+. A significantly elevated level of IL-4 (1697 pg/ml) was observed in the culture supernatant from CS-IIA. In addition, the conversion of phenotype from some CD4+CD8+ cells to CD4+CD8− was demonstrated by the neutralization assay using anti-IL-4 antibody. CS-IIA had a normal human karyotype and was free from Epstein-Barr virus nuclear antigen and immunoreactive with sera of HTLV-I- or HTLV-II-infected patients and anti-HTLV-1, p19 or p24 mAb. The provirus genome of HTLV-II was detected in this cell line by the polymerase chain reaction combined with a digoxigenin-enzyme-linked immunosorbent assay. However, electron microscopy of CS-IIA cells revealed no C-type virus particles in the extracellular space. These results indicate that HTLV-II can be transmitted from an HTLV-II-infected simian leukocyte cell line to human cord T lymphocytes and suggest that co-expression of CD4 and CD8 on T cells may be induced by the high level of IL-4, which can mediate CD8 induction on CD4+ T cell clones.

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Abbreviations

HTLV-II:

human-T-lymphotropic virus type II

PCR:

polymerase chain reaction

IL-2:

human interleukin-2

IL-4:

human interleukin-4

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Authors and Affiliations

  1. Second Department of Pathology, Okayama University Medical School, 2-5-1, Shikata-cho, 700, Okayama, Japan

    Kazuhiko Hayashi, Nobuya Ohara, Kotaro Fujiwara, Hiroyuki Aoki, Ho Jong Jeon, Kiyoshi Takahashi & Tadaatsu Akagi

  2. Okayama Red Cross Blood Center, 700, Okayama, Japan

    Noriko Tomita

  3. School of Health Sciences, Okayama University, 700, Okayama, Japan

    Kanji Miyamoto

Authors
  1. Kazuhiko Hayashi
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  2. Nobuya Ohara
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  3. Kotaro Fujiwara
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  4. Hiroyuki Aoki
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  5. Ho Jong Jeon
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  6. Kiyoshi Takahashi
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  7. Noriko Tomita
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  8. Kanji Miyamoto
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  9. Tadaatsu Akagi
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Hayashi, K., Ohara, N., Fujiwara, K. et al. Co-expression of CD4 and CD8 associated with elevated interleukin-4 in a cord T cell line derived by cocultivating normal cord leukocytes and an HTLV-II-producing simian leukocyte cell line (Si-IIA). J Cancer Res Clin Oncol 119, 137–141 (1993). https://doi.org/10.1007/BF01229527

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  • DOI: https://doi.org/10.1007/BF01229527

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