Summary
Islets were isolated from pancreases of 2-month and 12-month-old rats, and the oxidation of14C-glucose to14CO2 determined at various medium D-glucose concentration. Islets from 12-month-old rats oxidized significantly less glucose than those from 2-month-old rats at glucose concentrations of 150, 300, and 450 mg/dl, and this was true when islets were selected by hand or by Ficoll density gradient separation. The effect of age on glucose oxidation was seen when islets were incubated with [U-14C], [1-14C], or [6-14C] glucose. The results raise the possibility that previously reported age-related defects in glucose-stimulated insulin secretion may be secondary to the effect of age on islet glucose catabolism.
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Reaven E, Gold G, Reaven G (In press) Effect of age on glucose-stimulated insulin release by theβ cell of the rat. J Clin Invest
Lacy PE, Kostianovsky M (1967) Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16: 35–39
Rodbell M (1964) Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J Biol Chem 239: 375–380
Cole EH, Logothetopoulos J (1974) Glucose oxidation and insulin secretion by pancreatic islets isolated from hyperglycemic and normoglycemic rats. Diabetes 23: 469–473
Ammon HPT, Verspohl E (1976) Pyridine nucleotides in pancreatic islets during inhibition of insulin release by exogenous insulin. Endocrinology 99: 1469–1476
Akhtar MS, Phil M, Verspohl E, Hegner D, Ammon HPT (1977) 6-phosphogluconate/glucose-6-phosphate ratio in rat pancreatic islets during inhibition of insulin release by exogenous insulin. Diabetes 26: 857–863
Zawalich WS (1979) Intermediary metabolism and insulin secretion from isolated rat islets of Langerhans. Diabetes 28: 252–260
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Reaven, G.M., Reaven, P.D. Effect of age on glucose oxidation by isolated rat islets. Diabetologia 18, 69–71 (1980). https://doi.org/10.1007/BF01228306
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DOI: https://doi.org/10.1007/BF01228306