Skip to main content
Log in

Sulfhydryl-containing cells of the central nervous system

  • Published:
Acta Neurovegetativa Aims and scope Submit manuscript

Summary

Brains of several species were sectioned and stained by theBarrnett andSeligman technique for localizing sulfhydryl groups. Most of the cells of the brain react weakly, but in the phylogenetically older parts, e. g., hypothalamus, amygdaloid nucleus, and cingulate gyrus, certain astrocytes and microglia were observed which were highly reactive. They were observed in all species studied; they are not present at birth in the rat, but develop afterwards. Electrical stimulation of the cortex did not change their staining characteristics. The localization and possible significance of these cells was discussed.

Zusammenfassung

Hirnschnitte verschiedener Tierarten wurden nachBarrnett undSeligman gefärbt um Sulfhydrylgruppen zu lokalisieren. Die meisten Hirnzellen lassen sich nur schwach färben, aber in den, phylogenetisch gesehen, älteren Teilen, z. B. dem Hypothalamus, dem Nucleus amygdalae und dem Gyrus cinguli, sind bestimmte Astrocyten und Mikroglia zu beobachten, die sehr stark gefärbt erscheinen. Diese wurden bei allen untersuchten Tierarten festgestellt. Bei der Ratte sind sie bei der Geburt nicht zu finden, entwickeln sich aber später. Ihre Affinität zu verschiedenen Farbstoffen ändert sich nicht durch elektrischen Reiz des Cortex. Die Lokalisation und mögliche Bedeutung dieser Zellen wird besprochen.

Résumé

Des cerveaux de plusieurs espèces furent coupés et colorés selon la méthode deBarrnett etSeligman afin de localiser des groupements sulfhydryles. La plupart des noyaux cérébraux ne se colorent que faiblement, mais dans les parties philogéniquement plus vieilles comme par exemple dans l'hypothalamus, le faisceau de l'ourlet, la circonvolution, il y a quelques astrocytes et microglies donnant une réaction très vive. On les rencontrait dans toutes les espèces étudiées, ils ne sont pas présents dans les rats nouveau-nés, mais se développent plus tard. La stimulation électrique de l'écorce ne changeait pas leur réaction envers des colorants. La localisation et le rôle probable de ces noyaux sont évalués.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Barrnett, R., andA. M. Seligman, Histochemical Demonstration of Protein-Bound Sulfhydryl Groups. Science116 (1952), 323–327.

    PubMed  Google Scholar 

  2. Barrnett, R. J., andA. M. Seligman, Histochemical Demonstration of Sulfhydryl and Disulfide Groups of Protein. J. Nat. Cancer Inst., Wash.,14 (1954), 769–803.

    Google Scholar 

  3. Barrnett, R., andA. J. Ladman, Histochemical Demonstration of Protein Bound Sulfhydryl and Disulfide Groups in Cells of the Anterior Pituitary. Endocrinology54 (1954), 355–360.

    PubMed  Google Scholar 

  4. Barrnett, R. J., andA. M. Seligman, Histochemical Experiments on Sulfhydryls and Disulfides, in Glutathione. Academic Press, 89–101, New York, 1954.

    Google Scholar 

  5. Barron, E. S. G., Thiol Groups of Biological Importance. Advances Enzymol., N. Y.,11 (1951), 201–266.

    Google Scholar 

  6. Bertelli, A., andE. Mussini, Azione della Strofantina sui Gruppi-SH del Cuore. Bell. Soc. ital. biol. Spor.31 (1955), 1168–1171.

    Google Scholar 

  7. Hellerman, L., K. A. Schellenberg andO. K. Reiss, L-Glutamic Dehydrogenase, II, Role of Enzyme Sulfhydryl Groups. J. Biol. Chem., Baltimore,233 (1958), 1468–1478.

    Google Scholar 

  8. Roston, S., Interaction of Hydrocortisone, The Sulfhydryl Group, and the Catecholamines. Nature191 (1961), 490–491.

    PubMed  Google Scholar 

  9. Scheuch, D., C. Kohrig, E. Ockel, C. Wagenknecht andS. M. Rapoport, Role of Glutathione and of a Self-stabilizing Chain of SH-Enzymes and Substrates in the Metabolic Regulation of Erythrocytes. Nature190 (1961), 631–632.

    PubMed  Google Scholar 

  10. Sloper, J. C., andB. C. King, Activity in Secretory Neurones of the Hypothalamus and Posterior Pituitary of the Rat. J. Path. Bact.86 (1963), 179–198.

    PubMed  Google Scholar 

  11. Shelley, W. B., andR. Florence, Ethylene Clycol Monomethyl Ether, a New Fixative For Histological Work. Nature191 (1961), 719–720.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

With 4 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ellman, G.L., Sullivan, C.V. Sulfhydryl-containing cells of the central nervous system. Acta Neurovegetativa 27, 184–189 (1965). https://doi.org/10.1007/BF01226875

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01226875

Keywords

Navigation