Summary
The effect of diazoxide and gliclazide upon45calcium efflux from perifused islets was investigated. In the absence of extracellular calcium and presence of glucose, diazoxide caused an immediate and sustained increase in45calcium efflux. This facilitation of the outward transport of calcium might explain why diazoxide inhibits the stimulant action of glucose upon calcium accumulation in the beta cell. Under conditions known to abolish insulin release and the concomitant release of45calcium, namely in the absence of extracellular calcium or in the presence of deuterium oxide, gliclazide caused an immediate but transient decrease in45calcium efflux, this inhibition of the outward transport of calcium being observed both in the absence and presence of glucose and being followed by a significant rebound in45calcium efflux. It is suggested that this biphasic change in45calcium efflux could be due to a dual effect of gliclazide upon calcium handling by the beta cell, similar to that evoked by glucose and theophylline respectively.
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Malaisse, W.J.: The role of calcium in insulin secretion. Israel J. Med. Sci.8, 244–251 (1972).
Malaisse-Lagae, F., Malaisse, W.J.: The stimulussecretion coupling of glucose-induced insulin release. III. Uptake of45calcium by isolated islets of Langerhans. Endocrinology88, 72–80 (1971).
Malaisse, W.J., Brisson, G.R., Baird, L.E.: The stimulus-secretion coupling of glucose-induced insulin release. X. Effect of glucose on45calcium efflux from perifused islets. Amer. J. Physiol., in press.
Brisson, G.R., Malaisse-Lagae, F., Malaisse, W.J.: The stimulus-secretion coupling of glucose-induced insulin release. VII. A proposed site of action for adenosine-3′,5′-cyclic monophosphate. J. clin. Invest.51, 232–241 (1972).
Brisson, G.R., Malaisse, W.J.: The stimulus-secretion coupling of glucose-induced insulin release. XI. Effects of theophylline and epinephrine on 45calcium efflux from perifused islets. Metabolism, in press.
Malaisse, W.J., Leclercq-Meyer, V.: Insulinotropic action of a new sulfonylurea: gliclazide. Rev. Europ. Etudes Clin. et Biol.17, 310–314 (1972).
Malaisse, W.J., Malaisse-Lagae, F., Walker, M.O., Lacy, P.E.: The stimulus-secretion coupling of glucose-induced insulin release. V. The participation of a microtubular-microfilamentous system. Diabetes20, 257–265 (1971).
Malaisse, W.J., Mahy, M., Brisson, G.R., Malaisse-Lagae, F.: The stimulus-secretion coupling of glucose-induced insulin release. VIII. Combined effects of glucose and sulfonylureas. Europ. J. clin. Invest.2, 85–90 (1972).
Pipeleers, D.G., Marichal, M., Malaisse, W.J.: Metabolic, cationic and pharmacological influences on insulin biosynthesis. Diabetologia, in press.
Brisson, G.R., Malaisse, W.J.: Insulinotropic effect and possible mode of action of a new potent sulfonylurea (BS-4231). Canad. J. Physiol. Pharmacol.49, 536–544 (1971).
Malaisse, W. J., Malaisse-Lagae, F.: Effects of thiazides upon insulin secretion in vitro. Arch. int. Pharmacodyn.171, 235–239 (1968).
Malaisse, W.J., Malaisse-Lagae, F., Brisson, G.R.: Combined effects of glucose and sulfonylureas on insulin secretion by the rat pancreas in vitro. In: Recent hypoglycemic sulfonylureas, p. 114–128, ed. by U.C. Dubach and A. Bückert. Bern: Hans Huber 1971.
Rosen, O.M., Hirsch, A.H., Goren, E.N.: Factors which influence cyclic AMP formation and degradation in an islet cell tumor of the Syrian Hamster. Arch. Biochem.146, 660–663 (1971).
Goldfine, I.D., Perlman, R., Roth, J.: Inhibition of cyclic 3′,5′-AMP phosphodiesterase in islet cells and other tissues by tolbutamide. Nature234, 295–297 (1971).
Pace, C.S., Price, S.: Electrical activity of islet cells in response to leucine and tolbutamide. Diabetes21, 345 (1972).
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Malaisse, W.J., Pipeleers, D.G. & Mahy, M. The stimulus-secretion coupling of glucose-induced insulin release XII. Effects of diazoxide and gliclazide upon45calcium efflux from perifused islets. Diabetologia 9, 1–5 (1973). https://doi.org/10.1007/BF01225992
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DOI: https://doi.org/10.1007/BF01225992