Summary
This paper presents a rapid and sensitive method for the determination of carboxyhaemoglobin in blood. Carbon monoxide is released from blood by acid treatment and the gas is reacted with palladium chloride. The excess, unreacted palladium in its divalent state is estimated by its reaction with chlorpromazine hydrochloride, with which it forms a violet coloured complex with an absorption maximum at 565 nm. The sensitivity of the reaction between palladium and chlorpromazine hydrochloride allows levels of carboxyhaemoglobin below 10% to be estimated with precision. Only 0.1 ml to 0.5 ml of blood are required.
Zusammenfassung
Eine rasche und empfindliche Methode zur Bestimmung von Kohlenoxidhämoglobin im Blut wurde beschrieben. Das Kohlenmonoxid wird durch Ansäuern aus dem Blut freigesetzt und mit PdCl2 zur Reaktion gebracht. Das nicht verbrauchte Pd(II) wird mit Hilfe von Chlorpromazinchlorhydrat bestimmt, womit es einen violett gefärbten Komplex bildet, dessen Absorptionsmaximum bei 565 nm liegt. Die Empfindlichkeit dieser Farbreaktion gibt die Möglichkeit, CO-Hämobglobingehalte unter 10% genau zu bestimmen. Man benötigt dazu nur 0,1 bis 0,5 ml Blut.
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Lee, K.T., Kit, O.A. & Jacob, E. Determination of carboxyhaemoglobin in blood. Mikrochim Acta 64, 657–663 (1975). https://doi.org/10.1007/BF01225627
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DOI: https://doi.org/10.1007/BF01225627