Abstract
Rapid ethanol fermentation is defined as a fermentation in which the ethanol content increases from 0 to 94.8 g 1−1 in 6 horless. To achieve this by the fermentation of glucose withZymomonas mobilis, the initial biomass concentration must be at least 20 g dry wt 1−1 and that of the substrate must not exceed 150 to 200 g 1−1 during fermentation. The best results were obtained with a medium containing initielly 16% of the total sugar with the remaining glucose being added continuously, after 20 min of incubation, over 5 h at a rate of 0.21 g/min. After 6 h, ethanol reached 102 g 1−1, the volumetric productivity was 17g ethanol 1−1 h−1 and the yield 79.8 or 88% of the theoretical value, calculated according to the total fed or the consumed glucose, respectively.
Résumé
Quand la concentration d'alcool produit par fermentation monte de 0 a 94.8 g 1−1 dans un delai de 6 h ou moins, on appelle a cette fermentation, alcoolique rapide. Dans le present travail on a determiné les conditions pour avoir une fermentation alcoolique rapide a partir de glucose, utilisant une souche deZymomonas mobilis. On a trouvé que la concentration initiale de biomasse doit etre au moins 20 g de céllules poids sec/litre et la concentration du sucre doit se mantenir au dessous de 150–200 g 1−1 pendant la fermentation. Les meilleurs résultats qu'on a eu ont été avec un milieu qu'avait au dessous de 150–200 g 1−1 pendant la fermentation. Les meilleurs résultats qu'on a eu ont été avec un milieu qu'avait au commencement 1/6 du sucre total et l'excedent a été ajouté apres 20 minutes pendant 5 heures en quantités de 0.21 g/min. Aux 6 h la concentration d'alcool estarrivé a 102 g 1−1, le rendement calculé sur le sucre utilisé était 88% du théorique (79.8% du sucre alimenté) et la production volumétrique 17 g ethanol 1−1 h−1.
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Garro, O.E., Rodríguez, E. & Callieri, D.A.S. Experimental approaches to a rapid ethanol fermentation of glucose by aZymomonas mobilis strain. World J Microbiol Biotechnol 6, 67–72 (1990). https://doi.org/10.1007/BF01225358
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DOI: https://doi.org/10.1007/BF01225358