Abstract
Rhodotorula glutinis degraded variously14C-labelled synthetic lignins in the presence of 0.1% glucose as co-substrate. Side chain-labelled DHP was degraded the most. While this yeastutilized vanillate and forulate for growth, sinapate/syringate were poorly or not degraded. Gallate, protocatechuate and acetate also supported growth. [carboxy-14C]Syringate was rapidly converted to14CO2 by the yeast only in presence of glucose while [carboxy-14C]vanillate did not require any additional cosubstrate for mineralization. Ring-labelled vanillyl alcohol was also dagraded proving that the yeast could rapidly metabolize guaiacyl structures while syringyl structures required the presence of additional energy sources.
Résumé
Rhodotorula glutinis dégrade des lignines synthétiques marquées au14C de manière variée, en présence de 0.1% de glucose comme co-substrat. La DHP marquée sur la chaîne latérale est dégradée le plus. Alors que cette levure utilise le vanillate et le férulate pour sa croissance, le sinapate et le syringate sont peu ou prou dégradables. Le gallate, le protocatéchuate et l'acétate supportent également la croissance. Le syringate marqué au14C dans sa fonctioncarboxyle estrapidement convert en14CO2 par le levure mais exclusivement en présence de glucose, tandis que le vanillate marqué au14C dans sa fonction carboxyle ne requiert aucun co-substrat additionel pour sa minéralisation. L'alcool vanillique marqué dans son cycle, est également dégradé démonstrant ainsi que la levure peut métaboliser rapidement des structures guaiacyliques tandis que les structures syringiques requièrent la présence de sources auxiliaires d'énergie.
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Gupta, J.K., Sharma, P., Kern, H.W. et al. Degradation of synthetic lignins and some lignin monomers by the yeastRhodotorula glutinis . World J Microbiol Biotechnol 6, 53–58 (1990). https://doi.org/10.1007/BF01225355
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DOI: https://doi.org/10.1007/BF01225355