Abstract
A comparison of different habitats, biogas plant, rumen fluid and sewage sludge, for cellulolytic organisms indicated sewage studge was the best source. Enrichment cultura gave a mixed culture which exhibited CMCase activity as well as extracellular Avicelase, xylanase, β-glucosidase, β-xylosidase activities and cell-bound β-glucosidase, and β-xylosidase production in a synthetic medium with eleven different cellulosic and lignocellulosic substrates. The activity of extracellular β-glucosidase and β-xylosidase production was significantly higher than endogenous activities. Hemicellulases were induced better than cellulases. The anzyme system was stable under aerobic conditions. Of the different lignocellulosic substrates, kallar grass was the best inducer of extracellular enzymes.
Résumé
La comparaison de différents habitats: digesteur méthanique, fluide du rumen ou boue de station d'épuration, pour leur contenu en organismes cellulolytiques, indiquent que la boue de station d'épuration est la meilleure source. Une culture par enrichissement a produit une culture mixte qui a exhibé aussi bien une activité CMCase que des activitiés extracellulaires avicelasique, xylanasique, β-glucosidasique et β-xylosidasique et qu'une production de β-glucosidase et de β-xylosidase liées à la cellule, dans un milieu synthétique et pour onze substrats cellulosiques et lignocellulosiques différents. L'activité de la β-glucosidase extracellulaire et la production de β-xylosidase sont significativement plus élevées que les activitiés endogènes. Les hemicellulases sont mieux induites que les cellulases. Le système enzymatique est stable dans des conditions aérobies. Parmi les divers substrats lignocellulosiques, l'herbe Kallar est le meilleur inducteur d'enzymes extracellufaires.
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Tabassum, R., Rajoka, M.I. & Malik, K.A. Production of cellulases and hemicellulases by an anaerobic mixed culture from lignocellulosic biomass. World J Microbiol Biotechnol 6, 39–45 (1990). https://doi.org/10.1007/BF01225353
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DOI: https://doi.org/10.1007/BF01225353