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A cytochemical method for the identification of ischemic and necrotic myocardial fibres

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Summary

The author studied the nickel-dimethylglioxim reaction in ischemic and necrotic myocardium fibres in dogs, following ligature of the left anterior descending coronary artery. The nickel-dimethylglioxim reaction was positive in ischemic fibers, but not in necrotic ones. The cytochemical reaction, observed in ischemic fibers, was localized in the intracellular compartment and in the mitochondria.

Zusammenfassung

Untersucht wurde die Nickel-Dimethylglioxim-Reaktion in ischämischen und nekrotischen Herzmuskelzellen von Hunden. Vorausgegangen war eine Ligatur des linken absteigenden Koronararterienastes. Die Nickel-Dimethylglioxim-Reaktion war positiv in ischämischen Zellen, jedoch nicht in nekrotischen. Die cytochemische Reaktion, wie sie in ischämischen Fasern beobachtet wird, war im intrazellulärem Compartment und in den Mitochondrien lokalisiert.

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References

  1. Balogh I, Felszeghy E (1987) The possible role of endogenous nickel release in injured patients. 6th. International Catecholamine Symposium, Jerusalem 14–19.06.1987

  2. Balogh I, Somogyi E, Rubányi G (1982) Nickel cytochemistry. Applicability of a new cytochemical technique in forensic medicine. Acta Med Leg Soc 32:459–464

    Google Scholar 

  3. Balogh I, Somogyi E, Sótonyi P, Pogátsa G, Rubányi G, Bellus E (1983) Electroncytochemical detection of endogenous nickel in the myocardium in acute carbon monoxide poisoning. Z Rechtsmed 90:7–14

    Google Scholar 

  4. Felszeghy E, Balogh I (1986) Intramyocardial nickel accumulation and/or release in acute myocardial infarction. In: Anke M, Baumann W, Braunlich H, Brückner Chr, Groppel B (eds) Spurenelemente Al, As, Cd, Hg, Ni, Pb, Sn, Tl, Si, V. University Verlag, Leipzig Jena 3:1196–1200

    Google Scholar 

  5. Kohlhardt M, Wais U (1979) Quantitative differences between the inhibitory action of Verpamin and Ni ions on the slow response action potential in mammalian ventricular myocardium. J Mol Cell Cardiol 11:917–921

    Google Scholar 

  6. Kohlhardt M, Mnich Z, Haap K (1979) Analysis of the inhibitory effect of Ni ions on slow inward current in mammalian ventricular myocardium. J Mol Cell Cardiol 11:1227–1243

    Google Scholar 

  7. Kovach AGB, Balogh I, Rubányi G (1986) Subcellular localization of nickel ion in the myocardium. In: Basu S, Millette JR (eds) Electron microscopy in forensic, occupational and environmental health sciences. Plenum Press, New York London, pp 203–215

    Google Scholar 

  8. Leach CN Jr, Linden JV, Hopfer SM, Chrisostomo MC, Sunderman FW (1985) Nickel concentrations in serum of patients with acute myocardial infarction of unstable angina pectoris. Clin Chem 31:556–560

    Google Scholar 

  9. Lie JT (1968) Detection of early myocardial infarction by the acid fuchsin staining technique. Am J Clin Pathol 50:317–319

    Google Scholar 

  10. Lie JT, Holley KE, Kampa WE, Titusa JL (1971) New histochemical method for morphological diagnosis of early stage of myocardial ischemia. Mayo Clin Proc 46:319–327

    Google Scholar 

  11. McNeely MD, Sunderman FW Jr, Nechay MW, Levine H (1971) Abnormal concentrations of nickel in serum in cases of myocardial infarction, stroke, burns, hepatic cirrhosis and uremia. Clin Chem 17:1123–1128

    Google Scholar 

  12. Rubányi G, Balogh I, Somogyi E, Kovach AGB, Sótonyi P (1980) Effect of nickel ions on ultrastructure of isolated perfused rat heart. J Mol Cell Cardiol 12:609–618

    Google Scholar 

  13. Rubányi G, Szabó K, Balogh I, Bakos M, Gergely A, Kovach AGB (1980) Endogenous nickel release as a possible cause of coronary vasoconstriction and myocardial injury in acute burn of rats. Cite Shock 10:361–370

    Google Scholar 

  14. Rubányi G, Ligeti L, Koller A (1981) Nickel is released from the ischemic myocardium and contracts coronary vessels by a Ca-dependent mechanism. J Mol Cell Cardiol 13:1023–1026

    Google Scholar 

  15. Somogyi E, Balogh I, Sótonyi P, Kerényi N (1983) Comparative electronmicroscopic investigation of postmortem human heart muscle biopsy. Am J Forensic Med Pathol 4:7–13

    Google Scholar 

  16. Sunderman EW Jr, Nomoto S, Pradhan AM (1970) Increased concentration of serum nickel after acute myocardial infarction. N Engl J Med 283:986–999

    Google Scholar 

  17. Szabó K, Balogh I (1985) Endogenous nickel release in injured patients: a possible cause of myocardial damage. Injury 16:613–620

    Google Scholar 

  18. Van Reemps J, Borgers M, Reneman RS (1976) Early myocardial ischemia: evaluation of the histochemical haematoxylin-basic fuchsin-picric acid (HBFP) staining technique. Cardiovasc Res 10:262–267

    Google Scholar 

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Felszeghy, E. A cytochemical method for the identification of ischemic and necrotic myocardial fibres. Int J Leg Med 106, 148–151 (1993). https://doi.org/10.1007/BF01225237

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  • DOI: https://doi.org/10.1007/BF01225237

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