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Evaluation of a method for simultaneous quantification of codeine, dihydrocodeine, morphine, and 6-monoacetylmorphine in serum, blood, and postmortem blood

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Summary

A solid-phase extraction and gas chromatographic-mass spectrometric method for the simultaneous determination of codeine, dihydrocodeine, morphine, and 6-monoacetylmorphine in serum, blood or postmortem blood is described. The extraction technique allows the determination of free or total morphine (morphine plus morphine glucuronide). Experiments with spiked blood samples resulted in recoveries of 96.4% ± 4.2% for codeine, 95.8% ± 5.1% for dihydrocodeine, 90.3% ± 7.8% for 6-monoacetylmorphine and 92.5% ± 8.1% for morphine. Excellent linearity was obtained over the range 1–1500 ng/mL. The detection limit for all analytes is less than 1 ng/mL.

Zusammenfassung

Eine Festphasen-Extraktion und GC/MS-Methode zur gleichzeitigen Bestimmung von Codein, Dihydrocodein, Morphin und 6-Monoacetylmorphin in Serum, Blut und Leichenblut wird beschrieben. Die Extraktions-Technik ermöglicht die Bestimmung von freiem und Gesamtmorphin (Morphin plus Morphinglucuronid). Versuche mit aufgestockten Blutproben führten zu Wiederfindungsraten von 96.4% ±4.2% für Codein, 95.8% ±5.1% für Dihydrocodein, 90.3% ±7.8% für 6-Monoacetylmorphin und 92.5% ±8.1% für Morphin. Linearität wurde über den Konzentrationsbereich von 1–1500 ng/mL erhalten. Die Nachweisgrenzen liegen für alle Substanzen unter 1 ng/mL.

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Authors and Affiliations

  1. Institut für Rechtsmedizin, Heinrich-Heine-Universität Düsseldorf, Moorenstrasse 5, D-40001, Düsseldorf, Germany

    F. Mußhoff & Th. Daldrup

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  1. F. Mußhoff
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  2. Th. Daldrup
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Mußhoff, F., Daldrup, T. Evaluation of a method for simultaneous quantification of codeine, dihydrocodeine, morphine, and 6-monoacetylmorphine in serum, blood, and postmortem blood. Int J Leg Med 106, 107–109 (1993). https://doi.org/10.1007/BF01225050

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  • DOI: https://doi.org/10.1007/BF01225050

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