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Ultrastructural observations on the cytoarchitecture of axons processed by rapid-freezing and freeze-substitution

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Journal of Neurocytology

Summary

The structure and organization of axons in the cervical connective of wild-typeDrosophila fruit flies were examined in anticipation of studies of various neurological mutants. Dissected flies were rapid-frozen from the living state against a copper block cooled with liquid helium, freeze-substituted, and prepared for electron microscopic examination of thin sections. These cryotechniques showed new details of the structure of cell organelles and cytoplasm inDrosophila axons. The cytoplasmic matrix of axons and glia consists of a material with a fine granular texture enmeshed in a three-dimensional meshwork of short, fine filaments which vary in shape, size and electron density. No neurofilaments are present, but bundles of microtubules are interwoven into the filamentous matrix of the axoplasm. The round wall of microtubules (27 nm overall diameter) is composed of twelve cylindrical protofilaments with a typical substructural periodicity. Mitochondria frequently make contact with microtubules in both axons and glial processes. A thin layer of electron-dense filamentous matrix, which appears to be an axonal basal lamina, contacts most of the axonal exoplasmic surface, especially that of axons where they are surrounded by processes of glial cells, but is scant wherever single axons are contiguous. Thus, an axonal basal lamina occupies the constricted spaces around axons, where extracellular K+ accumulates during neural activity.

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  1. Gadi Benshalom

    Present address: Unit of Morphology, Corob Center for Health Sciences, Ben-Gurion University of the Negev, 84105, Beer Sheva, Israel

Authors and Affiliations

  1. Laboratory of Neurobiology, NINCDS, NIH at the Marine Biological Laboratory, 02543, Woods Hole, Massachusetts, USA

    Gadi Benshalom & Thomas S. Reese

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  1. Gadi Benshalom
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  2. Thomas S. Reese
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Benshalom, G., Reese, T.S. Ultrastructural observations on the cytoarchitecture of axons processed by rapid-freezing and freeze-substitution. J Neurocytol 14, 943–960 (1985). https://doi.org/10.1007/BF01224806

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  • DOI: https://doi.org/10.1007/BF01224806

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