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Determination of oxygen uptake by tissue grown in an all glass differential microrespirometer

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Summary

An all-glass differential microrespirometer is described, which is designed to measure the respiration of actively growing cellsin vitro. The actual growing of the cells was carried outin situ in the respirometer to avoid injury by a transfer of the living cells.

The apparatus was also used in measuring the oxygen uptake of bacterial cells and brain homogenate.

Zusammenfassung

Ein ganz aus Glas hergestelltes Differential-Mikrorespirometer wird beschrieben, das sich zur Messung der Atmung in vitro lebhaft wachsender Zellen eignet. Die Zellen werden im Respirometer in situ gezüchtet, um eine Verletzung der lebenden Zellen durch Übertragung zu vermeiden. Der Apparat wurde auch zur Messung der Sauerstoffaufnahme von Bakterienzellen und homogenisiertem Gehirnbrei verwendet.

Résumé

Description d'un microrespiromètre différentiel prévu pour mesurer la respiration de cellules croissant activement in vitro. La croissance réelle des cellules se produit in situ, dans le respiromètre, afin d'éviter toute lésion susceptible d'être provoquée par leur transfert.

L'appareil a été également utilisé pour mesurer les échanges d'oxygène au niveau de cellules bactériennes et d'homogénéisat de cerveau.

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Author information

Author notes

  1. Benjamin W. Grunbaum

    Present address: 180 Lyon Laboratories, University of Minnesota, Minneapolis, USA

Authors and Affiliations

  1. Department of Biochemistry and Virus Laboratory, University of California, Berkeley 4, Cal., USA

    Benjamin W. Grunbaum, Benjamin V. Siegel, Arthur R. Schulz & Paul L. Kirk

Authors
  1. Benjamin W. Grunbaum
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  2. Benjamin V. Siegel
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  3. Arthur R. Schulz
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  4. Paul L. Kirk
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Additional information

This investigation was supported in part by grants from the National Institute of Health, U. S. Public Health Service, and the University of California Committee on Research.

Fellow of the National Foundation for Infantile Paralysis at the University of California, 1953–1954.

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Grunbaum, B.W., Siegel, B.V., Schulz, A.R. et al. Determination of oxygen uptake by tissue grown in an all glass differential microrespirometer. Mikrochim Acta 43, 1069–1075 (1955). https://doi.org/10.1007/BF01224009

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  • DOI: https://doi.org/10.1007/BF01224009

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