Summary
The study was designed to show whether there was any relation between muscle capillary basement membrane thickness, HLA-antigens, antiinsulin antibodies and proliferative retinopathy. Electron microscopic measurements of muscle capillary basement membrane thickness were performed on muscle biopsies from 15 insulin-dependent diabetics with severe proliferative retinopathy, 24 insulindependent diabetics with minimal retinopathy and 18 age-and sex matched non-diabetics. All the patients had had diabetes for 20 years or more. None had biochemical or clinical evidence of diabetic nephropathy. Basement membrane thickness was measured according to the methods of Siperstein and Williamson. Muscle capillary basement membrane thickening occurred in 32 of 39 diabetics, using the Siperstein method, but patients with proliferative retinopathy did not exhibit thicker basement membranes than patients with no or minimal changes in the retina. There were apparrent differences in HLA-antigens between diabetics with and without proliferative retinopathy, but they did not reach statistical significance. There was no correlation between muscle capillary basement membrane thickness and the quantity of insulin antibodies. The results indicate that factors other than basement membrane thickening and genetic factors in the HLA-region, are responsible for the development of proliferative retinopathy.
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Williamson, J.R., Kilo, C.: Current status of capillary basement membrane disease in diabetes mellitus. Diabetes26, 65–73 (1977)
Østerby, R.: Early phases in the development of diabetic glomeruloparthy. Acta Med. Scand. (Suppl.)574, 1–80 (1975)
Thomsen, Å. C.: The kidney in diabetes mellitus. Thesis, pp. 240–242. Copenhagen: Munksgaard 1965
Watkins, P.J., Blainey, J.D., Brewer, D.B., Fitzgerald, M.G., Malins, J.M., O'Sullivan, D.J., Pinto, J.A.: The natural history of diabetic renal disease. Q. J. Med.164, 437–456 (1972)
Natvig, H.: Nye høide — vekttabeller for norske kvinner og menn. Oslo: Landsforeningen for kosthold og helse 1956
Andersen, O.O., Brunfeldt, K., Abildgård, F.: A method for quantitative determination of insulin antibodies in human plasma. Acta Endocrinol. (Kbh.)69, 195–208 (1972)
Kissmeyer-Nielsen, F., Kjerbye, K.E.: Lymphocytotoxic microtechnique. Purification of lymphocytes by flotation. Histocompatibility testing. Curtoni, E.S.V., Mattievz, P.L., Tosi, R.M. (Eds.): pp. 381–383, Copenhagen: Munksgaard 1967
Nielsen, L. Staub, Jersild, C., Ryder, L.P., Svejgaard, A.: HLA antigen, gene, and haplotype frequencies in Denmark. Tissue Antigens6, 70–76 (1975)
Siperstein, M.D., Unger, R.H., Madison, L.L.: Studies of muscle capillary basement membranes in normal subjects, diabetic and prediabetic patients. J. Clin. Invest.47, 1973–1999 (1968)
Williamson, J.R., Vogler, N.J., Kilo, C.: Estimation of vascular basement membrane thickness. Diabetes18, 567–578 (1969)
Beauchemin, M.L., Antille, G., Leuenberger, P.M.: Capillary basement membrane thickness: A comparison of two morphometric methods for its estimation. Microvasc. Res.10, 76 (1975)
Andersen, O.O.: The immunogenic properties of highly purified insulin preparations. The clinical importance of insulin-binding antibodies. Acta Endocrinol. (Kbh.)78, 723–735 (1975)
Nerup, J., Platz, P., Andersen, O.O., Christy, M., Lyngsøe, J., Poulsen, J.E., Ryder, L.P., Nielsen, L.S., Thomsen, M., Svejgaard, A.: HLA antigens and diabetes mellitus. Lancet1974 II, 864–866
Palmberg, P.F.: Diabetic retinopathy. Diabetes26, 703–709 (1977)
Larkins, R.G., Martin, F.I.R., Tait, B.D.: HLA patterns and diabetic retinopathy. Br. Med. J.1978 I, 1111
Möller, E., Persson, B., Sterky, G.: HLA phenotypes and diabetic retinopathy. Diabetologia14, 155–158 (1978)
Becker, B., Shin, D.H., Burgess, D., Kilo, C., Miller, W.V.: Histocompatibility antigens and diabetic retinopathy. Diabetes26, 997–999 (1977)
Pyke, D.A., Tattersall, R.B.: Diabetic retinopathy in identical twins. Diabetes22, 613–618 (1973)
Bertrams, J., Jansen, F.K., Grüneklee, D., Reis, H.E., Drost, H., Beyer, J., Gries, F.A., Kuwert, E.: HLA-antigens and immunresponsiveness to insulin in insulin-dependent diabetes mellitus. Tissue Antigens8, 13–17 (1976)
Andersen, O.O., Egeberg, J.: The clinical significance of insulin antibodies. Acta Paediatr. Scand. [Suppl.]270, 63–68 (1977)
Paetkau, M.E., Boyd, T.A.S., Winship, B., Grace, M.: Cigarette smoking and diabetic retinopathy. Diabetes26, 46–49 (1977)
Svejgaard, A., Ryder, L.P.: Associations between HLA and Disease — Notes on methodology and a report from the HLA and disease registry. In: HLA and disease. Dausset, J., Svejgaard, A. (Eds.), pp. 46–71. Copenhagen: Munksgaard 1977
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Deckert, T., Egeberg, J., Frimodt-Møller, C. et al. Basement membrane thickness, insulin antibodies and HLA-antigens in long standing insulin dependent diabetics with and without severe retinopathy. Diabetologia 17, 91–96 (1979). https://doi.org/10.1007/BF01222208
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DOI: https://doi.org/10.1007/BF01222208