Summary
The present report describes a novel approach for the identification of human or non-human specimens after long-term storage in a badly preserved state. The application of the PCR-technique (polymerase chain reaction) using human-specific primers as well as Southern blot (filter) hybridization of the sample DNA to a primate-specific DNA probe enabled us to extend the positive identification beyond the limits of conventional methods such as serological or morphological examinations.
Zusammenfassung
Der vorliegende Bericht beschreibt eine neuartige Strategie für die Identifizierung von menschlichen oder nicht-menschlichen Geweben nach Langzeitlagerung in schlecht erhaltenem Zustand. Die Anwendung der PCR-Technik (Polymerase Kettenreaktion) mit Hilfe humanspezifischer Starter sowie mit Hilfe der Southern Blot-Hybridisierung der Proben-DNA mit einer Primaten-spezifischen DNA-Sonde versetzte uns in die Lage, die positive Identifizierung jenseits der Nachweisgrenzen konventioneller Methoden, wie serologische und morphologische Untersuchungen auszudehnen.
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Müllenbach, R., Makuch, D., Wagner, HJ. et al. Application of DNA filter hybridization and PCR to distinguish between human and non-human tissues of poor quality. Int J Leg Med 105, 307–309 (1993). https://doi.org/10.1007/BF01222112
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DOI: https://doi.org/10.1007/BF01222112