Summary
A method for the prolonged incubation of adipose tissuein vitro is described. — Adipose tissue of normal, alloxan diabetic and fasted-refed rats survivesin vitro for 24 hours and remains responsive to insulin. — The glucose uptake of diabetic adipose tissue rises during 8 to 16 hours of incubationin vitro to values which are on the same order as those of normal tissue. This effect is independent of insulin. — Basal glycerol release rises during incubation. The glycerol release of the tissue is stimulated by insulin at the beginning of the incubation and inhibited at the end of incubation. — The comparison of the stimulation of intracellular glucose metabolism by prolonged incubation and by insulin may permit one to distinguish insulin effects on glucose transport from other possible effects on glucose metabolism. — The mechanisms responsible for the insulin-independent glucose penetration during prolonged incubation are presently being investigated.
Résumé
Une méthode permettant l'incubationin vitro de longue durée du tissu adipeux est décrite. — Ainsi le tissu adipeux épididymaire du rat survitin vitro jusqu'à 24 heures. — L'étude a porté sur le tissu adipeux de rats normaux, réalimentés après jeûne et alloxanisés. — Pendant toute la durée de l'incubation, le tissu répond à la stimulation par l'insuline. — Le métabolisme du glucose du tissu adipeux «diabétique», d'abord abaissé, augmente au cours de l'incubation à des valeurs comparables au tissu adipeux «normal»», ceci notamment en l'absence d'insuline. — La production de glycérol par le tissu augmente pendant l'incubation. L'insuline a une action stimulatrice sur la lipolyse au début, mais une action inhibitrice à la fin de l'incubation. — La comparaison entre l'action stimulatrice d'une incubation de longue durée sur le métabolisme intracellulaire du glucose et celle de l'insuline permettra de séparer l'action de l'insuline sur le transport du glucose de possibles actions supplémentaires de cet hormone dans la normalisation du métabolisme du tissu adipeux «diabétique». — Les causes de la stimulation du métabolisme du glucose lors d'une incubation sont examinées actuellement.
Zusammenfassung
Eine Methode der langfristigen Inkubation von epididymalem Fettgewebe von Ratten wird beschrieben. — Fettgewebe normaler, alloxandiabetischer und nach Nahrungskarenz widergefütterter Ratten überlebt in vitro während 18 bis 24 Stunden. Das Gewebe läßt sich während der ganzen Zeit mit Insulin stimulieren. Die verlängerte Inkubation von Fettgewebe alloxandiabetischer Ratten führt in den ersten 8 bis 16 Stunden ohne Insulin zu einem Anstieg der Glucoseaufnahme auf Werte, die denjenigen des normalen Gewebes entsprechen. — Die basale Glycerinabgabe steigt während der Inkubation an. Zu Beginn der Inkubation wird die Glycerinabgabe durch Insulin gefördert, gegen Ende der Inkubation teilweise gehemmt. — Der Vergleich des intracellulären Stoffwechsels markierter Glucose unter Insulinstimulation und nach langfristiger Inkubation dürfte einen Beitrag zur Beantwortung der Frage leisten, ob Insulin zusätzliche transportunabhängige Wirkungen auf den Glucosestoffwechsel des Fettgewebes ausübt. — Die Gründe der insulinunabhängigen Steigerung der Glucoseaufnahme während der langzeitigen Inkubation werden zur Zeit untersucht.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literatur
Beigelman, P.M., andP.B. Hollander: Effect of insulin upon resting electrical potential of adipose tissue. Proc. Soc. exp. Biol.110, 590 (1962).
Carruthers, B.M., andA.I. Winegead: Effects of insulin on amino acid and ribonucleic acid metabolism in rat adipose tissue. Amer. J. Physiol.202, 605 (1962).
Crofford, O.B., andA.E. Renold: Glucose uptake by incubated rat epididymal adipose tissue: rate limiting steps and site of insulin action. J. biol. Chem.240, 14 (1965).
— —: Glucose uptake by incubated rat epididymal adipose tissue: Characteristics of the glucose transport system and action of insulin. J. biol. Chem.240, 3237 (1965).
Eagle, H., V.I. Oyama, M. Levy andA.E. Freeman: Myo-Inositol as an essential growth factor for normal and malignant human cells in tissue culture. J. biol. Chem.226, 191 (1957).
Earle, W.R.: Production of malignancy in vitro; mouse flbroblast cultures and changes seen in living cells. J. nat. Cancer Inst.4, 165 (1943).
Folch, J., M. Lees andG.H. Sloane Stanley: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem.226, 497 (1957).
Frerichs, H., andE.G. Ball: Studies on the metabolism of adipose tissue. XL Activation of phosphorylase by agents which stimulate lipolysis. Biochemistry1, 501 (1962).
Froesch, E.R.: Fructose metabolism in adipose tissue from normal and diabetic rats. In:Renold, A. E., andG.F. Cahill, Jr., Eds., Handbook of Physiology, Section 5, Adipose Tissue, p. 281. Washington, D.C.; American Physiological Society, 1965.
—,H. Bürgi, E.B. Ramseier, P. Bally andA. Labhart: Antibody-suppressible and nonsuppressible insulinlike activities in human serum and their physiologic significance. An insulin assay with adipose tissue of increased precision and specificity. J. clin. Invest.42, 1816 (1963).
— —P. Bally andA. Labhart: Insulin inhibition of spontaneous adipose tissue lipolysis and effects upon fructose and glucose metabolism. Mol. Pharmacol.1, 280 (1965).
Galton, D. J., andFain, J.N.: Effects of prolonged incubation of isolated fat cells on their response to hormones stimulating lipolysis and glucose metabolism. Biochem. J.98, 557 (1966).
Gellhorn, A., andW. Benjamin: The intracellular localization of an enzymatic defect of lipid metabolism in diabetic rats. Biochim. biophys. Aeta84, 167 (1964).
Goodman, H.M.: Effects of insulin on water uptake by adipose tissue during incubationin vitro. Endocrinology76, 531 (1965).
—: Stimulatory action of insulin on leucine uptake and metabolism in adipose tissue. Amer. J. Physiol.206, 129 (1964).
Gordon, R. S., Jr.: Unesterified fatty acids in human blood plasma. II. The transport function of unesterified fatty acids. J. clin. Invest.36, 810 (1957).
Gromova, K. G.: Activity of hexokinase and glucokinase of adipose tissue in epididymis of rats and its regulation by insulin. Vop. Med. Him.10, 631 (1964).
Herrera, M.G., andA.E. Renold: Hormonal effects on glycine metabolism in rat epididymal adipose tissue, Biochim. biophys. Acta44, 165 (1960).
—,G.R. Philipps andA.E. Renold: Stimulation of metabolic activity of adipose tissue from fasted rats by prolonged incubationin vitro. Biochim. biophys. Acta106, 221 (1965).
Jungas, R.L., andE.G. Ball: Studies on the metabolism of adipose tissue: XVIII. In vitro effects of insulin upon the metabolism of the carbohydrate and triglyceride stores of adipose tissue from fasted-refed rats. Biochemistry3, 1696 (1964).
Martin, D.B., andP.R. Vagelos: The mechanism of tricarboxylic acid regulation of fatty acid synthesis. J. biol. Chem.237, 1787 (1962).
Leonards, J.R., andB.R. Landau: A study of the equivalence of metabolic patterns in rat adipose tissue: Insulin versus glucose concentration. Arch. Biochem.91, 194 (1960).
Philipps, G.R., andA.E. Renold, zitiert in Herrera, M.G., et al. Biochim. biophys. Acta106, 221 (1965).
Renold, A.E., O.B.Crofford, H.Bürgi and E.R.Froesch: Insulin and the metabolism of adipose tissue, 5th Congr. Intern. Diabetes Federation, Toronto, 1965. In: “On the Nature and Treatment of Diabetes (Wrenshall, G. A., and B. S.Leibel, Eds.) Excerpta Medica International Congress Series No. 84, p. 146.
Robinson, D.S.: The clearing factor lipase activity of adipose tissue. In:Renold, A. E., andG. F. Cahill, Jr., Eds., Handbook of Physiology, Section 5, adipose tissue, p. 295. Washington, D.C.; American Physiological Society, 1965.
Rodbell, M., andA.B. Jones: Metabolism of isolated fat cells: III. The similar inhibitory action of Phospholipase C and of insulin on lipolysis stimulated by lipolytic hormones and theophilline. J. biol. Chem.241, 140 (1966).
Salaman, M.R., Ph.D. thesis, Oxford, England, 1963.
Shafrir, E., andS. Kerpel: Fatty acid esterification and release as related to the carbohydrate metabolism of adipose tissue: effect of epinephrine, corticol and adrenalectomy. Arch. Biochem. Biophys.105, 237 (1964).
Vagelos, P.R., A.W. Alberts andD.B. Martin: Studies on the mechanism of activation of acetyl coenzyme-A carboxylase by citrate. J. biol. Chem.238, 533 (1963).
Winegrad, A.I.: Adipose tissue in diabetes, in:Renold, A.E., andG.F. Cahill, Jr., Eds., Handbook of Physiology, Section 5, adipose tissue, p. 319. Washington, D.C.; American Physiological Society, 1965.
Zierler, K.L., andD. Rabinowitz: Effect of very small concentrations of insulin on forearm metabolism. Persistance on its action on potassium and free fatty acids without its effects on glucose. J. clin. Invest.43, 950 (1964).
Author information
Authors and Affiliations
Additional information
Diese Arbeit erfolgte mit Unterstützung durch the United States Public Health Service (Grant No. A 5'387) und den Schweizerischen Nationalfonds (Nr. 3'854).
Rights and permissions
About this article
Cite this article
Meyer, U.A. Stoffwechsel des Fettgewebes normaler, alloxandiabetischer und nach Nahrungskarenz wiedergefütterter Ratten während langfristiger Inkubation in vitro. Diabetologia 2, 189–194 (1966). https://doi.org/10.1007/BF01222069
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01222069