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Colorimetric determination of microgram quantities of isonicotinic acid hydrazide (isoniazid)

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Summary

The orange yellow colour of the vanadium(V)-isoniazid complex measured at 420 nm (Filter No. 42) is utilized as the basis for the colorimetric determination of isoniazid in the pH range 1.8 to 2.2. Beer's law is obeyed in the range 0.67–13.6 ppm. The influence of the substrates that are commonly employed as excipients in pharmaceutical preparations has been studied. The reliability of the method was tested by applying to the determination of isoniazid in “Isokin” tablets. Results are in good agreement with those obtained by the official B. P. method.

Zusammenfassung

Die orange-gelbe Farbe des Vanadin(V)-Isoniazidkomplexes wird bei 420 nm (Filter Nr. 42) gemessen und dient zur kolorimetrischen Bestimmung von IsonikotinsÄurehydrazid bei pH 1,8–2,2. Zwischen 0,67 und 13,6 ppm ist das Beer'sche Gesetz erfüllt. Der Einflu\ von Substraten, die üblicherweise als ArzneimitteltrÄger dienen, wurde geprüft. Die Brauchbarkeit der Methode wurde an „Isokin“-Tabletten erprobt. Die Ergebnisse stimmen mit denen der offiziellen Methode der Brit. Pharmacop. gut überein.

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Authors and Affiliations

  1. Department of Chemistry, Andhra University, Waltair, India

    P. V. Krishna Rao, G. Bala Bhaskara Rao & P. Smaba Murti

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  1. P. V. Krishna Rao
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  2. G. Bala Bhaskara Rao
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  3. P. Smaba Murti
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Rao, P.V.K., Rao, G.B.B. & Murti, P.S. Colorimetric determination of microgram quantities of isonicotinic acid hydrazide (isoniazid). Mikrochim Acta 62, 979–984 (1974). https://doi.org/10.1007/BF01221875

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  • DOI: https://doi.org/10.1007/BF01221875

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