Summary
The intraaxonal transport of noradrenaline and tyrosine hydroxylase was studied in the sciatic nerves of diabetic, obese and appropriate control mice. Noradrenaline and tyrosine hydroxylase accumulated proximal to a constriction applied to the nerves. Noradrenaline concentration in the non-constricted sciatic nerves did not differ significantly in nondiabetic and diabetic mice (0.67±0.04 and 0.58±0.01 ng/cm respectively); in obese mice and lean littermates the noradrenaline concentration was 0.47±0.05 ng/cm and 0.46±0.01 ng/cm. After nine hours of constriction the concentration of noradrenaline increased in the axons of nondiabetic (1.15±0.06 ng/cm), in obese (0.90±0.08 ng/cm) and lean mice (1.10±0.07 ng/cm) but remained low (0.68±0.07 ng/cm) in diabetic mice. — Administration of insulin (10 U/kg/day) to diabetic mutants completely reversed the decrease in NA accumulation. NA accumulated only in the nerve segment proximal (1 cm) to a constriction and was transported distally at an apparent velocity of 0.75 mm/hr in control axons. The difference of NA accumulation between diabetic and nondiabetic control indicate a reduced rate of axoplasmic flow in the noradrenergic axons of diabetic animals.
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Giachetti, A. Axoplasmic transport of noradrenaline in the sciatic nerves of spontaneously diabetic mice. Diabetologia 16, 191–194 (1979). https://doi.org/10.1007/BF01219797
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DOI: https://doi.org/10.1007/BF01219797