Summary
The acitivity of five glycohydrolases was measured in the plasma of Chinese hamsters from eight highly inbred lines in the Upjohn colony. The diabetic animals showed elevated activities of plasmaβ-D-galactosidase, N-acetyl-β-D-glucosaminidase and N-acetyl-β-D-galactosaminidase but similar activities of plasmaα-D-glucosidase andα-D-galactosidase to the nondiabetic animals. Line-specific variation was observed in all five enzymes and anomalies were especially evident in N-acetyl-β-D-glucosaminidase and N-acetyl-β-D-galactosaminidase activity. In two diabetic lines, AC and Z, activities of these two enzymes were not elevated although significant correlation was found with blood sugar levels. The pronounced difference in the plasma activity of N-acetyl-β-D-glucosaminidase in two diabetic lines, XA and AC, did not involve plasma inhibitors or activators, as evidenced by the coincidence in observed and calculated activities in mixed plasma samples, or specific isozymes, concluded from the similar elution profiles on ionexchange column and thermostability curves. These data suggest that diabetes-related changes in plasma glycohydrolase activities are dictated by genetic factors and may be involved in the development of complications.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Belfiore, F., LoVecchio, L., Napoli, E., Borzi, V.: Increasedβ-N-acetyl-glucosaminidase activity in diabetes mellitus. Clin. Chem.20, 1229–1230 (1974)
Belfiore, F., Napoli, E., LoVecchio, L.: Serum N-acetyl-β-glucosaminidase activity in diabetic patients. Diabetes21, 1167–1172 (1972)
Bomback, F. M., Nakagawa, S., Kumin, S., Nitowsky, H. M.: Altered lysosomal glycohydrolase activities in juvenile diabetes mellitus. Diabetes25, 420–427 (1976)
Fushimi, H., Tarui, S.: Retina, tear, and serumβ-N-acetylglucosaminidase activities in diabetic patients. Clin. Chini. Acta71, 1–8 (1976)
Fushimi, H., Tarui, S.: Kidney and serumβ-N-acetylglucosaminidase activities in streptozotocin diabetic rats and their responses to insulin and glucagon. J. Biochem. (Tokyo)76, 225–227 (1974)
Tulsiani, D. R. P., Buschiazzo, H. O., Tolbert, B., Touster, O.: Changes in plasma hydrolase activities in hereditary and streptozotocin-induced diabetes. Arch. Biochem. Biophys.181, 216–227 (1977)
Chang, A. Y., Noble, R. E., Wyse, B. M.: Streptozotoc-ininduced diabetes in the Chinese hamster. Diabetologia13, 595–602 (1977)
Spiro, R. G.: Investigations into the biochemical basis of diabetic basement-membrane alterations. Diabetes25 [Suppl. 2], 909–913 (1976)
Kofoed, J. A., Bozzini, C. E., Alippi, R. M.: Skin acid glycosaminoglycans in alloxan diabetic rats. Diabetes19, 732–733 (1970)
Chang, A. Y.: Acid glycohydrolase in Chinese hamster with spontaneous diabetes. II. N-Acetyl-β-D-hexosaminidase in plasma and tissues. Biochim. Biophys. Acta522, 503–514 (1978)
Chang, A. Y., Noble, R. E., Wyse, B. M.: Comparison of highly inbred diabetic and nondiabetic lines in the Upjohn colony of Chinese hamsters. Diabetes26, 1063–1071 (1977)
Chang, A. Y., Noble, R. E., Greenberg, H. S.: Variance in LDH isozyme patterns in a Chinese hamster (Cricetulus griseus) colony. Comp. Biochem. Physiol. [B]58, 119–123 (1977)
Chang, A. Y., Greenberg, H. S.: Acid glycohydrolase in Chinese hamster with spontaneous diabetes. III. Line-dependent variance. Biochim. Biophys. Acta525, 134–141 (1978)
Wyse, B. M., Chang, A. Y., Greenberg, H. S.: Glucose, insulin and glucagon levels in nondiabetic and spontaneously diabetic Chinese hamsters. Diabetes27 [Suppl. 2], 514 (1978)
Gerritsen, G. C., Dulin, W. E.: Effect of a new hypoglycemic agent, 3,5-dimethylpyrazole, on carbohydrate and free fatty acid metabolism. Diabetes14, 507–515 (1965)
Robinson, D., Price, R. G., Dance, N.: Separation and properties ofβ-galactosidase,β-glucosidase,β-glucoronidase and N-acetyl-glucosaminidase from rat kidney. Biochem. J.102, 525–532 (1967)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chang, A.Y., Perry, C.S. Acid glycohydrolase in Chinese hamster with spontaneous diabetes. Diabetologia 15, 423–429 (1978). https://doi.org/10.1007/BF01219653
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01219653