Summary
Administration of a hypoglycaemic sulphonamide, gliclazide, at 10 mg/kg/day p. o. to rabbits for 60 days did not affect the development of plasma lipid disturbances induced by a high cholesterol diet. The accumulation of cholesterol in the liver was significantly reduced by up to 34% when compared with animals on the high cholesterol diet. The high concentrations of glycerides and fatty acids in the aorta were significantly decreased towards normal values and histology showed that the gliclazide strongly inhibited the development of aortic and particularly coronary lesions induced by the atherogenic diet. A normal appearance of coronary arteries was noted in more than 50% of cases.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
University Group Diabetes Program: A study of the effects of hypoglycaemic agents on vascular complications in patients with adult-onset diabetes: I—Design, methods and baseline results. Diabetes19 (Suppl. 2), 747–783 (1970)
University Group Diabetes Program: A study of the effects of hypoglycaemic agents on vascular complications in patients with adult-onset diabetes: II — Mortality results. Diabetes19 (Suppl. 2), 787–830 (1970)
Albrink, M.J.: The microtitration of total fatty acids of serum with notes on the estimation of triglycerides. J. Lipid Res.1, 53–59 (1959)
Delsal, J.L., Manhouri, M.: Etude comparative des dosages colorimétriques du phosphore. Recherche d'une méthode de haute sensibilité applicable au dosage du phosphore organique dans les spots après Chromatographie. Bull. Soc. Chim. Biol.9–10, 1041–1054 (1955)
Desnoyers, P., Labaume, J., Baudet, M., Verry, M.: Fibrinolytic activity of vascular endothelium, demonstrated by Todd's method after administration of a new antidiabetic drug: 1702 SE. 2nd Congress of the International Society of Thrombosis and Haemostasis, 12–16 th July, 1971, Oslo
Desnoyers, P., Labaume, J., Anstett, M., Herrera, M., Pesquet, J., Sebastien, J.: The pharmacology of 1702 SE, a new highly effective antidiabetic drug with unusual properties. III —Antistickiness activity fibrinolytic properties and haemostatic parameters study. Arzneim. Forsch.22, 1691–1695 (1972)
Duhault, J., Desnoyers, P., Bérégi, L., Hugon, P.: Pharmacological approaches to the treatment of diabetes. Diabetologia6, 43 (Abstract) 1970
Duhault, J., Lebon, F., Bérégi, L.: Protective action of 1702 SE on the microvascular system in the normal and diabetic rats. Diabetes20 (Suppl. 1), 353 (1971)
Ericksson, S.: Influence of thyroid activity on excretion of bile acids and cholesterol in the rat. Proc. Soc. Exp. Biol. Med.94, 582–584 (1957)
Fiske, C.H., Subbarow, Y.: The coloric determination of phosphorus. J. Biol. Chem.66, 375–400 (1925)
Fletcher, K., Myant, N.B.: Influence of the thyroid on the synthesis of cholesterol by liver and skin in vitro. J. Physiol. (Lond.)144, 361–372 (1958)
Folch, J., Lees, M., Stanley, G.H. S.: A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem.226, 497–509 (1957)
Gendre, P.: Evolution de l'ultrastructure de l'atherome cholesterique experimental chez le lapin. Pathol. Eur.4, 235–256 (1969)
Hanno, G., Awwad, H.K.: A study of the antithyroid effect of tolbutamide. Endocrinology25, 343–350 (1962)
Hartel, G.: Über die Bedeutung der Schilddrüse für das Auftreten von erhöhten Serumcholesterinwerten bei Ratten nach Fütterung von N1-sulfanilyl-N2-n-butylcarbamid (BZ 55) Acta Endocrinol. (Kbh.)29, 267–270 (1958)
Hartel, G., Punsar, S., Louhija, A.: Die Wirkung hoher Dosen von N1sulfanilyl-N2-n-butylcarbamid auf die Regression experimenteller Atherosklerose, auf spontane Atherosklerose und auf Serumcholesterinwerte bei Hähnchen. Arzneim. Forsch.8, 301–304 (1958)
Havel, R.J., Felts, J.M., Van Duyne, C.M.: Formation and fate of endogenous triglycerides in blood plasma of rabbits. J. Lipid Res.3, 297–308 (1962)
Kampelmann, F.: Über die Wirkung von arseniger Säure auf die Schilddrüse und den Hypophysenvorderlappen. Arch. Exp. Pathol. Pharmakol.184, 139–151 (1937)
Keen, G., Jarrett, R.J.: Tolbutamide prophylaxis of arterial disease in borderline diabetics. Diabetes17 (Suppl. 1), 328–332 (1968)
Landriscina, C., Gnoni, G.V., Quagliariello, E.: Fatty acids biosynthesis: Physiological role of the elongation system present in microsomes and mitochondria of rat liver. Eur. J. Biochem.29, 188–196 (1972)
Laurell, S., Tibbling, G.: Colorimetric microdetermination of free fatty acids in plasma. Clin. Chim. Acta16, 57–62 (1966)
Marquié, G.: Effets préventifs du gliclazide sur le développement de l'athérosclerose induite par le cholestérol chez le lapin. C. R. Acad. Sci. [D] (Paris)175, 197–200 (1977)
Marquié, G., Agid, R.: Effets de certains sulfamides hypoglycémiants sur le développement de l'athérome expérimental induit par le cholestérol chez le lapin. J. Physiol. (Paris)59, 262 (1967)
Marquié, G., Agid, R.: Effets d'un sulfamide hypoglycémiant sur la régression des troubles lipidiques et l'évolution des lésions artérielles chez le lapin après cessation du régime riche en cholestérol. C. R. Soc. Biol. (Paris)165, 192–196 (1971)
Marquié, G., Agid, R.: Influence d'un régime riche en cholestérol sur l'activité du pancréas endocrine du lapin. Gen. Comp. Endocrinol.18, 113 A (1972)
Marquié, G., Agid, R.: Effets de la metformine (NN-diméthylbiguanide) sur l'hypothyroidie et l'infiltration lipidique du cortex surrénal, induites par le régime cholestérolé chez le lapin. Pathol. Biol. (Paris)21, 133–139 (1973)
Miller, B.F., Kothari, H.V.: Increased activity of lysosomal enzymes in human atherosclerotic aortas. Exp. Mol. Pathol.10, 288–294 (1969)
Paasikivi, J., Wahlberg, F.: Preventive tolbutamide treatment and arterial disease in mild hyperglycaemia. Diabetologia7, 323–327 (1971)
Punsar, S., Hartel, G.: Über den Einfluß von N1-n-sulfanilyl-N2-n-butylcarbamid auf die experimentelle Atherosklerose bei Hähnchen. Ann. Med. Exp. Biol. Fenn.35, 189–197 (1957)
Somogyi, M.: Notes on sugar determination. J. Biol. Chem.195, 19–23 (1952)
Smit-Sibinga, C. T., Wieringa, R. A.: The effect of gliclazide on irradiation induced experimental atheromatosis. 1st World Congress on Microcirculation, 18th June 1975, Toronto, Canada. Proceedings in press —Plenum Press, N. Y.
Sperry, W.M., Brand, F.: Determination of total lipids in blood serum. J. Biol. Chem.213, 69–76 (1955)
Verry, M., Desnoyers, P., Thouverez, J. P.: Activité anti-adhesive plaquettaire d'un nouvel antidiabétique, le 1702 SE. 2nd Mediterranean Congress on Thromboembolism, 11–14th October 1971, Istanbul
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marquié, G. Preventive effect of gliclazide on experimental atherosclerosis in rabbits. Diabetologia 14, 269–275 (1978). https://doi.org/10.1007/BF01219427
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01219427