Summary
The adenylate cyclase activities in 1,500 × g to 25,000 × g sediments of adipose tissue from 3 month old C57BL 6J/ob and lean mice were compared. Three anomalies were observed in obese mice: 1) a defect in basal (−80%) and fluoridestimulated (−50%) activities suggesting a decreased number of catalytic units per mg protein; 2) an impairment in stimulation by isoproterenol (−85%) with no change in apparent affinity for the hormone; and, 3) no stimulatory effect of ethanol upon guanyl nucleotide-stimulated activity. The two latter effects may be due to a reduced number ofβ-adrenergic receptors per mg protein and/or to an alteration in the transducing process. On the other hand, guanyl nucleotides as well as prostaglandin PGE1 exerted similar effects in the preparations from both lean and obese adipose tissue.
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Abbreviations
- ob/ob:
-
obese-hyperglycaemic
- cyclic AMP:
-
adenosine 3′,5′-cyclic monophosphate
- Gpp(NH)p:
-
guanyl-5′-yl imidodiphosphate
- PGE1 :
-
prostaglandin E1
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Dehaye, J.P., Winand, J. & Christophe, J. Adenylate cyclase activity in the epididymal adipose tissue from obese-hyperglycaemic mice. Diabetologia 15, 45–51 (1978). https://doi.org/10.1007/BF01219328
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DOI: https://doi.org/10.1007/BF01219328