Summary
An extremely sensitive hydrogen calorimeter has been developed for the purpose of determining the heats of hydrogenation of large molecules, particularly those of biochemical significance. High sensitivity is necessary because the substances to be studied are frequently expensive and difficult to purify. The present method has been evaluated by reproducing the known heats of hydrogenation of several isomers of hexene with a typical standard deviation and agreement with literature values of about 0.2 kcal/mole or less than 1 %. Typical sample size is in the range of 5 to 15 mg,
Zusammenfassung
Ein Wasserstoff-Kalorimeter besonderer Empfindlichkeit zur Bestimmung der Hydrierungswärme großer Moleküle, vor allem solcher von biochemischem Interesse, wurde entwickelt. Da die in Frage kommenden Stoffe teuer und auch schwer rein darstellbar sind, ist hohe Empfindlichkeit des Gerätes unerläßlich. Durch Bestimmung der aus der Literatur bekannten Hydrierungs wärme von etwa 0,2 Kcal/Mol verschiedener isomerer Hexene mit einer typischen Standardabweichung von weniger als 1% wurde das Verfahren überprüft. Einwaagen von 5–15 mg sind geeignet.
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Rogers, D.W., Papadimetriou, P.M. & Siddiqui, N.A. An improved hydrogen microcalorimeter for use with large molecules. Mikrochim Acta 64, 389–400 (1975). https://doi.org/10.1007/BF01219204
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DOI: https://doi.org/10.1007/BF01219204