Summary
A phototitrimetric method for the determination of sulfhydryl compounds in an aqueous medium is described.o-Hydroxymercuribenzoic acid is used as a titrant with dithiofluorescein as an indicator. Disulfide amino acids like cystine andα-substituted DL-cystines are reduced with sodium borohydride before titration. All amino acids were determined to the limit of 1.0μmol with an error of less than 4%. A selective phototitrimetric method for the determination ofβ-mercaptopyruvate in presence of cysteine and glutathione is described. This method can be extended to the microdetermination of other similar sulfhydryl compounds in biological materials.
Zusammenfassung
Eine phototitrimetrische Bestimmungsmethode für Sulfhydrylverbindungen in wäßriger Phase wurde beschrieben. Als Maßflüssigkeit diento-Hydroxymercuribenzoesäure, als Indikator Dithiofluorescein. Aminosäuredisulfide wie Cystin undα-substituierte DL-Cystine werden vor der Titration mit Natriumborhydrid reduziert. Alle Aminosäuren lassen sich bis zur Größenordnung von 1,0μMol mit einem Fehler von weniger als 4% bestimmen. Eine selektive phototitrimetrische Bestimmung vonβ-Merkaptopyruvat in Gegenwart von Cystein und Glutathion wurde angegeben. Das Verfahren läßt sich auch für andere Sulfhydrylverbindungen in biologischem Material anwenden.
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Ip, M.P.C., Thibert, R.J. Phototitrimetric determination of sulfhydryl compounds witho-hydroxymercuribenzoic acid using dithiofluorescein as an indicator. Mikrochim Acta 65, 599–612 (1976). https://doi.org/10.1007/BF01218236
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DOI: https://doi.org/10.1007/BF01218236