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Microchemical investigations of medicinal plants. XIV

Identification of the alkaloids in the leaves ofIpomoea violacea using preparative thin layer chromatography and solid probe mass spectrometry

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Summary

This paper presents a micromethod for the characterization of the alkaloids obtained from the Pearly Gates variety of the morning glory plant (Ipomoea violacea). Using 150μg of total alkaloid and mass spectrometry after thin layer chromatographic separation, the absolute identification of six alkaloids (lysergic acid amide,iso- lysergic acid amide, ergonovine, ergometrinine, argroclavine, elymoclavine) has been established. The proposed fragmentation patterns are described. The presence of another alkaloid, chanoclavine, in the leaves is discussed.

Zusammenfassung

Die Charakterisierung der aus Ipomoea violacea erhaltenen Alkaloide auf mikrochemischem Wege wurde beschrieben. 150μg Gesamtalkaloide wurden durch DC getrennt und massenspektrometrisch untersucht. Damit wurden 6 Alkaloide eindeutig erkannt, u. zw. Lysergsäureamid,i-Lysergsäureamid, Ergonovin, Ergometrinin, Argroclavin, Elymoclavin. Die vorgeschlagenen Bruchstückmuster wurden beschrieben. Die Anwesenheit eines weiteren Alkaloids Chanoclavin in den Blättern wurde diskutiert.

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References

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Author notes

  1. Jeffrey M. Weber

    Present address: Drug Enforcement Administration, Northeast Regional Laboratory, 555 W 57th Street, New York, N. Y.

Authors and Affiliations

  1. Department of Chemistry, City University of New York, 11210, Brooklyn, N. Y., USA

    Jeffrey M. Weber &  T. S. Ma

Authors
  1. Jeffrey M. Weber
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  2. T. S. Ma
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Additional information

For Part XIII see Mikrochim. Acta [Wien]1976 I, 217.

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Weber, J.M., Ma, T.S. Microchemical investigations of medicinal plants. XIV. Mikrochim Acta 65, 227–242 (1976). https://doi.org/10.1007/BF01217830

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  • DOI: https://doi.org/10.1007/BF01217830

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