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A simple rapid method for determining sucrose concentration in sucrose gradient centrifugation

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Summary

The red complex formed by hydroxyl groups with ceric ammonium nitrate served as a means for the rapid and direct determination of sucrose in sucrose gradient centrifugation both for analytical and preparative purposes. In the present work rabbit anti bovine serum albumin serum was separated into 7 S and 19 S components concentrating in the 17–20% sucrose and 26–28% sucrose range respectively.

Zusammenfassung

Die von Cerammoniumnitrat mit Hydroxylverbindungen unter Rotfärbung stattfindende Komplexbildung wurde zur raschen direkten Saccharosebestimmung bei der Rohrzucker- Gradientenzentrifugation für analytische und präparative Zwecke herangezogen. Im vorliegenden Fall wurde mit Antirinderserumalbumin sensibilisiertes Kaninchenserum in eine 7 S- und eine 19 S-Komponente getrennt, von denen erstere in 17- bis 20%igem Rohrzucker, letztere in 26- bis 28%igem Rohrzucker angereichert wurde.

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References

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Authors and Affiliations

  1. Department of Life Sciences, Division of Biochemistry, Bar-Ilan University, Ramat-Gan, Israel

    M. Rosenberg, M. Trop, R. Avtalion & A. Pinsky

Authors
  1. M. Rosenberg
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  2. M. Trop
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  3. R. Avtalion
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  4. A. Pinsky
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Rosenberg, M., Trop, M., Avtalion, R. et al. A simple rapid method for determining sucrose concentration in sucrose gradient centrifugation. Mikrochim Acta 59, 757–760 (1971). https://doi.org/10.1007/BF01217101

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  • DOI: https://doi.org/10.1007/BF01217101

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