Skip to main content
Log in

Semiquantitative determination of seven amino acids by the nichrome wire ring chamber

  • Published:
Microchimica Acta Aims and scope Submit manuscript

Summary

A method for microidentification and semiquantitative determination of L-arginine, L-(+) cysteine, L-histidine, DL-methionine, L-proline, DL-tryptophan and L-(−) tyrosine from a single drop of solution using the nichrome wire ring chamber is described. The accuracy is ± 5% and the method is selective and rapid. Other amino acids, vitamins and sugars do not interfere. The application of the method is demonstrated by the determination of amino acids from eggs, meat and “Procasenol”.

Zusammenfassung

Der Mikronachweis und die semiquantitative Bestimmung von L-Arginin, L-(+)-Cystein, L-Histidin, DL-Methionin, L-Prolin, DL-Tryptophan und L-Tyrosin in einem Tropfen Lösung mit Hilfe der Nichromdrahtringkammer wurde beschrieben. Die Genauigkeit beträgt ± 5%. Die Methode ist selektiv und rasch. Andere Aminosäuren, Vitamine und Zucker stören nicht. Die Anwendung des Verfahrens auf die Analyse von Hühnereiweiß, Fleisch und „Procasenol“ wurde beschrieben.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. M. B. Čelap, T. J. Janjić, andM. Ilić, Mikrochim. Acta [Wien]1962, 504.

  2. M. H. Hashmi, M. A. Shahid, N. A. Chughtai, andM. A. Rana, Mikrochim. Acta [Wien]1968, 1143.

  3. M. H. Hashmi, N. A. Chughtai, andM. A. Shahid, Mikrochim. Acta [Wien]1968, 1237.

  4. E. Stahl, Thin-layer Chromatography. New York: Academic Press. 1965. p. 408.

    Google Scholar 

  5. H. D. Springall, The Structural Chemistry of Proteins. London: Butterworths Scientific Publications. 1954. p. 238.

    Google Scholar 

  6. M. H. Hashmi, A. S. Adil, N. A. Chughtai, F. R. Chughtai, andM. A. Shahid, Mikrochim. Acta [Wien]1968, 291.

  7. H. Weisz, Microanalysis by the Ring Oven Technique. Oxford: Pergamon Press. 1961.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hashmi, M.H., Chughtai, N.A., Shahid, M.A. et al. Semiquantitative determination of seven amino acids by the nichrome wire ring chamber. Mikrochim Acta 57, 36–43 (1969). https://doi.org/10.1007/BF01216662

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01216662

Keywords

Navigation