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The simultaneous determination of cysteine and cystine using N-bromosuccinimide: Application in an enzymatic system—cystine reductase

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Summary

A new, rapid, convenient, and accurate method for the simultaneous determination of cysteine and cystine has been developed for use in pure solutions as well as in the enzymatic system, cystine reductase. N-Bromosuccinimide is the only standard solution employed in these determinations. Cysteine was determined at pH 7 by displacement of iodine from potassium iodide with N-bromosuccinimide. The total cysteine plus cystine was determined by N-bromosuccinimide using Bordeaux Red as an indicator. A comparative study to determine cysteine using N-bromosuccinimide and potassium iodate was performed.

It has been found that the values obtained with potassium iodate in the enzymatic system are relatively high. N-Bromosuccinimide gives more accurate results as shown by determining the residual amount of cystine after a particular time of incubation. When N-bromosuccinimide is used as a titrant for cysteine and/or cystine in pure solutions the error is less than ±1%. When N-bromosuccinimide is used as a titrant in the enzymatic system for the determination of cystine, the method is more precise and more accurate when compared to the use of potassium iodate.

Zusammenfassung

Ein Verfahren zur gleichzeitigen Bestimmung von Cystein und Cystin sowohl in reinen Lösungen wie im enzymatischen System der Cystinreduktase wurde ausgearbeitet. N-Bromsuccinimid (I) dient als einzige Standardlösung für solche Bestimmungen. Cystein wurde bei pH 7 auf der Basis der Jodfreisetzung aus Kaliumjodid mit I.bestimmt. Die Gesamtmenge Cystein + Cystin wurde mit I gegen Bordeaux-Rot als Indikator titriert.

Vergleichsweise wurde die Cysteinbestimmung mit I und mit Kaliumjodat durchgeführt. Im enzymatischen System ergaben sich mit Jodat relativ hohe Werte. I gibt genauere Resultate, wie sich durch Bestimmung der Restmenge Cystin nach einer bestimmten Inkubationszeit zeigen läßt. Wird I als Maßlösung zu Cystein und/oder Cystin in reinen Lösungen verwendet, so ist der Fehler kleiner als ±1 %. Verwendet man I zur Titration von Cystin im enzymatischen System, so erzielt man größere Genauigkeit als mit Kaliumjodat.

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Authors and Affiliations

  1. Department of Chemistry, University of Windsor, Windsor, Ontario, Canada

    R. J. Thibert, M. Sarwar & J. E. Carroll

Authors
  1. R. J. Thibert
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  2. M. Sarwar
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  3. J. E. Carroll
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Thibert, R.J., Sarwar, M. & Carroll, J.E. The simultaneous determination of cysteine and cystine using N-bromosuccinimide: Application in an enzymatic system—cystine reductase. Mikrochim Acta 57, 615–624 (1969). https://doi.org/10.1007/BF01216466

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  • DOI: https://doi.org/10.1007/BF01216466

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