Skip to main content
SpringerLink
Log in

Serum levels of interleukins, growth factors and anglogenin in patients with endometrial cancer

  • Original Paper
  • Clinical Oncology
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

The purpose of this work was to study changes in serum levels of interleukins, growth factors and angiogenin during different stages of endometrial cancer progression. Serum levels were assayed by enzyme-linked immunosorbant assay in 59 women with stages I–IV of endometrial cancer (study subjects: stage I,n=20; stage II,n=8; stage III,n=5; stage IV,n=6) and compared to the serum levels in 20 women without cancer as control subjects. Patients with endometrial cancer had varied serum levels of interleukins and growth factors. There was a significant increase in serum levels of angiogenin in all stages of tumor progression. Levels of interleukin-8 (IL-8), IL-10 and transforming growth factor β (TGFβ) were significantly elevated in patients with stages I and II carcinoma. The serum levels of tumor necrosis factor α (TNFα), granulocyte/macrophage-colony-stimulating factor, basic fibroblast growth factor (BFGF), IL-7 and IL-2 were significantly elevated in patients with stages II and III carcinoma and the serum level of tumor necrosis factor β (TNFβ) was slightly elevated in patients with stage II carcinoma only. The serum levels of IL-1α, IL-1β and IL-6 were not elevated in endometrial cancer patients in any of the clinical stages. The results showed that progression of endometrial cancer is associated with increased serum levels of cytokines, growth factors and angiogenin, which possibly amplify angiogenesis during different clinical stages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Alderson MR, Tough TW, Ziegler SF, Grabstein H (1991) Interleukin-7 induces cytokine secretion and tumoricidal activity by human peripheral blood monocytes. J Exp Med 173:923–930

    Google Scholar 

  2. Baiocchi G, Kavanagh JJ, Talpaz M, Wharton JT, Gutterman JU, Kurzrock R (1991) Expression of the macrophage colony-stimulating factor and its receptor in gynecologic malignancies. Cancer 67:990–996

    Google Scholar 

  3. Charnock-Jones DS, Sharkey AM, Rajput-Williams J, Burch D, Schofield JP, Fountain SA (1993) Identification and localization of alternately spliced mRNA for vascular endothelial growth factor in human uterus and estrogen regulation in endometrial carcinoma cell lines. Biol Reprod 48:1120–1128

    Google Scholar 

  4. Cozzolino F, Torcia M, Lucibello M, Morbidelli L, Ziche M, Platt J, Fabiani S, Brett J, Stern D (1993) Interferon-α and interleukin-2 synergistically enhance basic fibroblast growth factor synthesis and induce release, promoting endothelial cell growth. J Clin Invest 91:2504–2512

    Google Scholar 

  5. Dejana E, Bussolino F, Wang JM, Mantovani A (1991) Cytokine regulation of endothelial cells. In: F. R. Balkwell (ed) Cytokines, a practical approach. IRL London, pp 215–227

    Google Scholar 

  6. Detmar M, Tenorio S, Hettmannsperger U, Ruszczak Z, Orfanos CE (1992) Cytokine regulation of proliferation and ICAM-1 expression of human dermal microvascular endothelial cells in vitro. J Invest Dermatol 98:147–153

    Google Scholar 

  7. Folkman J (1985a) Tumor angiogenesis. Adv Cancer Res 43:175–203

    Google Scholar 

  8. Folkman J (1985b) Toward an understanding of angiogenesis: search and discovery. Perspect Biol Med 29:10–36

    Google Scholar 

  9. Folkman J, Klagsbrun M (1987a) Vascular physiology. A family of angiogenic peptides. Nature 329:671–672

    Google Scholar 

  10. Folkman J, Klagsburn M (1987b) Angiogenic factors. Science 235:442–447

    Google Scholar 

  11. Folkman J, Watson K, Ingber D, Hanahan D (1989) Induction of angiogenesis during the transition from hyperplasia to neoplasia. Nature 339:58–61

    Google Scholar 

  12. Fuchs A, Lindenbaum E, Maecoudas NG (1985) Endometrial angiogenesis. Acta Anat (Basel) 124:241–244

    Google Scholar 

  13. Grosen EA, Yamamoto RS, Ioli G, Ininns WK, Gatanaga M, Gatanaga T, DiSaia PJ, Berman M, Manetta A, Granger GA (1992) Blocking factors (soluble membrane receptors) for tumor necrosis factor and lymphotoxin detected in ascites and released in short-term cultures obtained from ascites and solid tumors in women with gynecologic malignancy. Lymphokine Cytokine 11:347–353

    Google Scholar 

  14. Grosen EA, Granger GA, Gatanaga M, Innins EK, Hwang C, DiSaia P, Berman M, Manetta A, Emma D, Gatanaga T (1993) Measurement of the soluble membrane receptors for tumor necrosis factor and lymphotoxin in the sera of patients with gynecologic malignancy. Gynecol Oncol 50:68–77

    Google Scholar 

  15. Horn DW, Vollmer G, Deerberg F, Schneider MR (1993) The EnDA endometrial adenocarcinoma: an estrogen-sensitive, metastasizing, in vivo tumor model of the rat. J Cancer Res Clin Oncol 119:450–456

    Google Scholar 

  16. Hu GF, Chang SI, Riordan JF, Vallee BL (1991) An angiogenin-binding protein from endothelial cells. Proc Natl Acad Sci USA 88:2227–2231

    Google Scholar 

  17. Irwin JC, Utian WH, Eckert RL (1991) Sex steroids and growth factors differentially regulate the growth and differentiation of cultured human endometrial stromal cells. Endocrinology 129:2385–2392

    Google Scholar 

  18. Klagsbrun M, DIAmore PA (1991) Regulators of angiogenesis. Annu Rev Physiol 53:217–239

    Google Scholar 

  19. Koch AE, Polverini PJ, Kunkel SL, Harlow LA, DiPietro LA, Elner VM, Elner SG, Strieter RM (1992) Interleukin-8 as a macrophage-derived mediator of angiogenesis. Science 258:1798–1801

    Google Scholar 

  20. Kristensen MS, Paludan K, Larsen CG, Zachariae CO, Deleuran BW, Jensen PK, Jorgensen P, Thestrup-Pedersen K (1991) Quantitative determination of IL-1α-induced IL-8 mRNA levels in cultured human keratinocytes, dermal fibroblasts, endothelial cells and monocytes. J Invest Dermatol 97:506–510

    Google Scholar 

  21. Leibovich SJ, Polverini PJ, Shepard HM, Wiseman DM, Shively V, Nuseir N (1987) Macrophage-induced angiogenesis is mediated by tumor necrosis factor-α. Nature 329:630–632

    Google Scholar 

  22. Moore KW, O'Garra A, de Waal Malefyt RW, Vieira P, Mosmann TR (1993) Interleukin-10. Annu Rev Immunol 11:165–190

    Google Scholar 

  23. Nelson KG, Takahashi T, Bossert NL, Walmer DK, McLachlan JA (1991) Epidermal growth factor replaces estogen in the stimulation of female genital-tract growth and differentiation. Proc.Natl Acad Sci USA 88:21–25

    Google Scholar 

  24. Pearl ML, Talavera E, Gretz HF III, Roberts JA, Menon KM (1993) Mitogenic activity of growth factors in the human endometrial adenocarcinoma cell lines HEC-1-A and KLE. Gynecol Oncol 49:325–333

    Google Scholar 

  25. Presta M (1988) Sex hormones modulate the synthesis of basic fibroblast growth factor in human endometrial adenocarcinoma cells: implications for the neovascularization of normal and neoplastic endometrium. J Cell Physiol 137:593–597

    Google Scholar 

  26. Presta M, Rusnati M, Maier JAM, et al (1991) Basic fibroblast growth factor and endothelial cells: receptor interaction, signal transduction, cellular response-dissociation of the mitogenic activity of β-FGF from its plasminogen activator inducing activator inducing capacity. In: Maragoudakis ME, Gullino P, Lelkes PI (eds) Angiogenesis in health and disease 227:63–78

  27. Soncin F (1992) Angiogenin supports endothelial and fibroblast cell adhesion. Proc Natl Acad Sci USA 89:2232–2236

    Google Scholar 

  28. Sunderkotter C, Goebeler M, Schulze-Osthoff K, Bhardwaj R, Sorg C (1991) Macrophage-derived angiogenesis factors. Pharmacol Ther 51:195–216

    Google Scholar 

  29. Weidner N, Carroll PR, Flax J, Blumenfeld W, Folkman J (1993) Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma. Am J Pathol 143:401–409

    Google Scholar 

  30. Wiseman DM, Polverini PJ, Kamp DW, Leibovich SJ (1988) Transforming growth factor-beta (TGF-β) is chemotactic for human monocytes and induces their expression of angiogenic activity. Biochem Biophys Res Commun 157:793–800

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Gynecological Oncology, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, 77555-0587, Texas, Galveston, USA

    Vimlarani Chopra, Tung V. Ding & Edward V. Hanningan

Authors
  1. Vimlarani Chopra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tung V. Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edward V. Hanningan
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work has been partially supported by a Gustavus and Louise Pfeiffer Research Foundation grant to Vimlarani Chopra

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chopra, V., Ding, T.V. & Hanningan, E.V. Serum levels of interleukins, growth factors and anglogenin in patients with endometrial cancer. J Cancer Res Clin Oncol 123, 167–172 (1997). https://doi.org/10.1007/BF01214669

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01214669

Key words

Search

Navigation