Aneuploidy in spermatozoa using fluorescence in situ hybridization

  • G. L. Schattman
  • S. Munné
  • J. G. Grifo
  • L. Carton
  • J. Cohen
News and Views Genetics


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Pearson PL, Bobrow M, Vosa CG: Technique for identifying Y chromosomes in human interphase nuclei. Nature 1970;226:78Google Scholar
  2. 2.
    Caspersson T, Zech L, Johannson C, Modest EJ: Identification of human chromosomes by DNA binding fluorescent agents. Chromosoma 1970;30:215Google Scholar
  3. 3.
    Barlow P, Vosa CG: The Y chromosome in human spermatozoa. Nature 1970;226:961Google Scholar
  4. 4.
    Wryobeck AJ, Gordon L, Burkhart J, Francis M, Kapp R Jr, Letz G, Malling H, Topham J, Whorton M: An evaluation of human sperm as indicators of chemically induced alterations of spermatogenic function. A report of the U.S. Environmental Protection Agency Gene-tox Program. Mutat Res 1983;115:73Google Scholar
  5. 5.
    Roberts AM, Goodall H: Y chromosome visibility in Quinacrine stained spermatozoa. Nature 1976;262:493Google Scholar
  6. 6.
    Sumner AT, Robinson JA: A difference in the dry mass between the heads of the X and Y bearing spermatozoa. J Reprod Fertil 1976;48:9Google Scholar
  7. 7.
    Pawlowitzki IH, Pearson PL: Chromosomal aneuploidy in human spermatozoa. Humangenetik 1972;46:119Google Scholar
  8. 8.
    Beatty RA: F-bodies as chromosome markers in mature human sperm heads. Cytogenet Cell Genet 1977;18:33Google Scholar
  9. 9.
    Rudak E, Jacobs PA, Yanagimachi R: Direct analysis of the chromosome constitution of human spermatozoa. Nature 1978;274:911Google Scholar
  10. 10.
    Brandriff B, Gordon L, Ashworth L, Watchmaker G, Carrano AV, Wryobeck AJ: Chromosomal abnormalities in human sperm: Comparisons among four healthy men. Hum Genet 1984;66:193Google Scholar
  11. 11.
    Brandriff B, Gordon L, Ashworth L, Watchmaker G, Moore II D, Wryobeck AJ, Carrano AV: Chromosomes of human sperm: Variability among normal individuals. Hum Genet 1985;70:18Google Scholar
  12. 12.
    Martin RH, Lin CC, Balkan W, Burns K: Direct chromosomal analysis of human spermatozoa: Preliminary results from 18 normal men. Am J Hum Genet 1982;34:459Google Scholar
  13. 13.
    Martin RH, Balkan W, Burns K, Rademaker AW, Lin CC, Rudd NL: The chromosome constitution of 1000 human spermatozoa. Hum Genet 1983;63;605Google Scholar
  14. 14.
    Martin RH, Rademaker AW, Hildebrand K, Long-Simpson L, Peterson D, Yamamoto J: Variation in the frequency and type of sperm chromosomal abnormalities among normal men. Hum Genet 1987;77:108Google Scholar
  15. 15.
    Martin RH, Rademaker AW: The frequency of aneuploidy among individual chromosomes in 6,821 human sperm chromosome complements. Cytogenet Cell Genet 1990;53:103Google Scholar
  16. 16.
    Estop AM, Cieply K, Vankirk V, Munne S, Garver K: Cytogenetic studies in human sperm. Hum Genet 1991;87:447Google Scholar
  17. 17.
    Genesca A, Benet J, Caballin MR, Germa JR, Egozcue J: Significance of structural chromosome abberations in human sperm: Analysis of induced abberations. Hum Genet 1990;85:495–499Google Scholar
  18. 18.
    Alikani M, Adler A, Reing A, Malter H, Cohen J: Subzonal sperm insertion and the frequency of gamete fusion. J Assist Reprod Genet 1992;9:97Google Scholar
  19. 19.
    Joseph AM, Gosden JR, Chandley AC: Estimation of aneuploidy levels in human spermatozoa using chromosome specific probes and in situ hybridization. Hum Genet 1984;66:234Google Scholar
  20. 20.
    Wryobeck AJ, Alhborn T, Balhorn R, Stanker L, Pinkel D: Fluorescence in situ hybridization to Y chromosomes in decondensed human sperm nuclei. Mol Reprod Dev 1990;27:1–9Google Scholar
  21. 21.
    Pieters MHEC, Geraedts JPM, Meyer H, Dumoulin JCM, Evers JLH, Jongbloed RJE, Nederlof PM, van der Flier S: Human gametes and zygotes studied by nonradioactive in situ hybridization. Cytogenet Cell Genet 1990;53:15Google Scholar
  22. 22.
    Guttenbach M, Schmid M: Determination of Y chromosomal aneuploidy in human sperm nuclei by nonradioactive in situ hybridization. Am J Hum Genet 1990;46:553Google Scholar
  23. 23.
    Han TL, Ford JH, Webb GC, Flaherty SP, Correll A, Mathews CD: Simultaneous detection of X and Y bearing human sperm by double fluorescence in situ hybridization. Mol Reprod Dev 1993;34:308Google Scholar
  24. 24.
    Jacobs PA, Browne C, Gregson N et al: Estimates of the frequency of chromosome abnormalities detectable in unselected newborns using moderate levels of banding. J Med Genet 1992;29(2):103–108Google Scholar
  25. 25.
    Jacobs PA: Chromosome abnormalities and fertility in man.In Proceedings International Symposium, the Genetics of Spermatozoon, Edinburgh, Beatty RA, Gluecksohn-Waelsch S (eds). 1971, pp 346–358Google Scholar

Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • G. L. Schattman
    • 1
  • S. Munné
    • 1
  • J. G. Grifo
    • 1
  • L. Carton
    • 1
  • J. Cohen
    • 1
  1. 1.The Gamete and Embryo Research LaboratoryThe Center for Reproductive Medicine and Infertility, Cornell University Medical CenterNew YorkUSA

Personalised recommendations