Advertisement

Radiation and Environmental Biophysics

, Volume 23, Issue 3, pp 213–222 | Cite as

Tumour-cord parameters in two rat hepatomas that differ in their radiobiological oxygenation status

  • J. V. Moore
  • H. A. Hopkins
  • W. B. Looney
Article

Summary

Tumour cords have been examined quantitatively in two rat hepatomas, 3924A and H-4-II-E, that differ in their radiobiological oxygenation status (oxygen enhancement ratio for growth delay [tumour clamped: tumor ‘in air’] was 1.35 for 3924A and only 1.08 for H-4-II-E). The average thickness of tumour cords in 3924A was 118 µm and only 69 µm in H-4-II-E. The migration rates across the cords of the two tumours were approximately the same (1.7 and 1.4 µm ⋅ h−1) but for any given distance from the subtending blood vessel, the proportion of histologically-dead cells within the cord was always higher for H-4-II-E. Volume for volume, H-4-II-E contained four times as much vascular space as 3924A but it is suggested that the poorquality of this vasculature in H-4-II-E contributed to its relative radioresistance.

Keywords

Oxygen Blood Vessel Migration Rate Average Thickness Oxygenation Status 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Aisenberg AC, Morris HP (1963) Energy pathways of hepatoma H-35 and 7800. Cancer Res 23: 566–568Google Scholar
  2. 2.
    Chalkley HW (1943) Method for the quantitative morphologic analysis of tissues. J Natl Cancer Inst 4: 47–53Google Scholar
  3. 3.
    Franko AJ, Sutherland RM (1979) Oxygen diffusion distance and development of necrosis in multicell spheroids. Radiat Res 79: 439–453Google Scholar
  4. 4.
    Hirst DG, Denekamp J (1979) Tumour cell proliferation in relation to the vasculature. Cell Tissue Kinet 12: 31–42Google Scholar
  5. 5.
    Hirst DG, Denekamp J, Hobson B (1982) Proliferation kinetics of endothelial and tumour cells in three mouse mammary carcinomas. Cell Tissue Kinet 15: 251–261Google Scholar
  6. 6.
    Jones B, Camplejohn RS (1983) Stathmokinetic measurement of tumour cell proliferation in relation to vascular proximity. Cell Tissue Kinet 16: 351–355Google Scholar
  7. 7.
    Looney WB, Hopkins HA, MacLeod MS (1979) The scheduling of treatment for a chemotherapeutically resistant experimental solid tumour. Cancer 43: 1201–1210Google Scholar
  8. 8.
    Looney WB, Hopkins HA, Trefil JS (1978) Perturbations in the kinetics of tumour and host organ cellular regulation demonstrated by single and combined experimental therapy. Adv Exp Med Biol 92: 677–757Google Scholar
  9. 9.
    Rowley R, Hopkins HA, Betsill WL, Ritenour ER, Looney WB (1980) Response and recovery kinetics of a solid tumour after irradiation. Br J Cancer 42: 586–595Google Scholar
  10. 10.
    Steel GG (1968) Cell loss from experimental tumours. Cell Tissue Kinet 1: 193–207Google Scholar
  11. 11.
    Sutherland RM, Eddy HA, Bareham B, Reich K, Vanantwerp D (1979) Resistance to adriamycin in multicellular spheroids. Int J Radiat Oncol Biol Phys 5: 1225–1230Google Scholar
  12. 12.
    Tannock IF (1968) The relation between cell proliferation and the vascular system in a transplanted mouse mammary tumour. Br J Cancer 22: 258–273Google Scholar
  13. 13.
    Tannock IF (1972) Oxygen diffusion and the distribution of cellular radiosensitivity in tumours. Br J Radiol 45: 515–524Google Scholar
  14. 14.
    Tannock IF, Steel GG (1970) Tumour growth and cell kinetics in chronically hypoxic animals. J Natl Cancer Inst 45: 123–133Google Scholar
  15. 15.
    Thomlinson RH, Craddock EA (1967) The gross response of an experimental tumour to single doses of X-rays. Br J Cancer 21: 108–123Google Scholar
  16. 16.
    Thomlinson RH, Gray LH (1955) The histological structure of some human lung cancers and the possible implications for radiotherapy. Br J Cancer 9: 539–549Google Scholar
  17. 17.
    Van Rossum GDV, Gosalvez M, Galeotti T, Morris HP (1971) Net movements of monovalent and bivalent cations, and their relations to energy metabolism, in slices of hepatoma 3924A and of a mammary tumour. Biochim Biophys Acta 245: 263–276Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • J. V. Moore
    • 1
  • H. A. Hopkins
    • 2
  • W. B. Looney
    • 2
  1. 1.Paterson LaboratoriesChristie Hospital and Holt Radium InstituteManchesterEngland
  2. 2.Division of Radiobiology and BiophysicsUniversity of Virginia Medical SchoolCharlottesvilleUSA

Personalised recommendations