Summary
The present experiments are an attempt to precise the type and localization of the cells involved in the protective effect of hemopoietic suspensions against the radiation-induced thymic lymphosarcoma (TLS) of C57BL/6 mice. Inocula containing variable numbers of BM or spleen CFUs from 60-day-old and 360-day-old donors were tested. According to their origin, the suspensions differed with respect to the CFU replication rate, the CFU ability to differentiate towards the T lineage and the content of the suspensions in thymic precursors. Two levels of inhibition were observed: BM suspensions from 60-day-old donors containing 1,500 CFUs had the best protective effect: 14.5% of TLS; 1,500 CFUs from 360-day-old donors were slightly but not significantly less efficient (28.5%). The second level of inhibition (36–46% of TLS) was obtained with all the following inocula:a) 1,200 and 300 spleen CFUs or 300 and 95 BM CFUs from 60-day-old donors,b) 1,500 spleen CFUs from aged donors. Seventy-six spleen CFUs from 60-day-old donors, 120 BM or 175 spleen CFUs from aged donors had no effect. These results suggest that in addition to the high replication rate of the BM CFUs as compared with spleen CFUs, cells endowed with an optimal protective effect are present in BM suspensions and are either absent or present in very small amount in spleen suspensions. These cells which induce an early repopulation of the thymus might correspond to thymic precursors.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abramson S, Miller RG, Phillips RA (1977) The identification in adult bone marrow of pluripotent and restricted stem cells of myeloid and lymphoid systems. J Exp Med 145: 1567–1579
Albrigth JW, Makinodan T (1976) Decline in the growth potential of spleen colonizing bone marrow stem cells of long lived aged mice. J Exp Med 114: 1204–1213
Boniver J, Decleve A, Lieberman M, Monsik C, Travis M, Kaplan HS (1981) Marrow-thymus interactions during radiation leukemogenesis in C57BL/Ka mice. Cancer Res 41: 390–392
Chazan R, Haran-Ghera N (1976) The role of thymus sub-populations in T cell development. Cell Immunol 23: 356–372
Duplan JF (1968) Etude comparative des cellules souches hématopoíétiques de la moelle osseuse et du foie foetal. Hématol 8: 445–456
Ford CE (1966) The use of chromosome markers. In: Micklem HS, Loutit JF (eds) Tissue grafting and radiation. Academic Press, New York London
Haot J, Betz EH, Simar LJ, Revesz L (1974) Origin, morphology and functionnal characteristics of a new lymphoid cell type in irradiated mouse bone marrow. Acta Haematol 51: 170–178
Haran-Ghera N, Peled A (1979) Induction of leukemia in mice by irradiation and radiation leukemia virus variants. Adv Cancer Res 30: 45–87
Jacobson LO, Simmons EL, Marks EK, Elbridge JH (1951) Recovery from radiation injury. Science 113: 510–511
Kaplan HS, Brown MB (1951) Further observations on inhibition of lymphoid tumor development by shielding and partial body-irradiation of mice. J Natl Cancer Inst 12: 427–432
Kaplan HS, Brown MB (1952a) Protection against radiation-induced lymphoma development by shielding and partial-body irradiation of mice. Cancer Res 12: 441–444
Kaplan HS, Brown MB (1952b) A quantitative dose response study of lymphoid tumor development in irradiated C57Black mice. J Natl Cancer Inst 13: 185–208
Kaplan HS, Brown MB, Paul L (1953) Influence of bone marrow injections on involution and neoplasia of mouse thymus after systemic irradiation. J Natl Cancer Inst 14: 303–316
Kaplan HS, Moses LE, Brown MB, Nagareda CS, Hirsch BB (1955) The time factor in inhibition of lymphoid tumor development by injection of marrow cell suspensions into irradiated C57BL mice. J Natl Cancer Inst 15: 975–979
Lahiri SK, Van Putten LM (1969) Distribution and multiplication of colony forming units from bone marrow and spleen after injection into irradiated mice. Cell Tissue Kinet 2: 21–28
Leonard A, Decleve A (1979) A chromosome marker for studies in the C57BL/6 strain of mice. Leuk Res 3: 93–98
Lorenz E, Uphoff D, Reid TR, Shelton E (1951) Modification of irradiation injury in mice and guinea-pig by bone marrow injection. J Natl Cancer Inst 12: 197–206
Lorenz E, Congdon CC, Uphoff D (1953) Prevention of irradiation induced lymphoid tumors in C57BL mice by spleen protection. J Natl Cancer Inst 14: 291–301
Micklem HS, Odgen DA, Payne AC (1973) Ageing haemopoietic stem cells and immunity. In: Haemopoietic Stem Cell. Ciba Foundation Symposium. Elsevier/North Holland, Amsterdam
Muramatsu S, Monnot P, Duplan JF (1976) Competitive proliferation in the hemopoietic tissues of irradiated hybrid mice engrafted with parental bone marrow and spleen. Exp Hematol 4: 188–200
Shisa H, Daculsi R, Duplan JF (1977) Production of thymic cells by mouse spleen and bone marrow. Biomedicine 27: 315–318
Till JE, McCulloch EA (1961) A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res 14: 213–222
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Legrand, E., Daculsi, R., Galiay, M. et al. Protective effect of bone marrow and spleen suspensions on radiation-induced leukemogenesis in C57BL/6 mice. Radiat Environ Biophys 23, 203–212 (1984). https://doi.org/10.1007/BF01213222
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01213222