Abstract
Effect of irradiation dose on the production of cellulase and amylase related enzymes inTrichoderma reesei was studied, in which post-irradiation time responce pattern was measured. The damage of the cells irradiated with certain irradiation doses (1.40±0.20x105, 2.20±0.10x105, 3.00±0.50 x 10 and 3.50±0.20 x 10 rad) was rapidly recovered. The increased enzyme production in the culture of the irradiated cells resulted from the recovery of radiation damage after irradiation. The function of cell growth was not affected by irradiation below dose of 5 x 105 rad, though the function of enzyme synthesis was drastically affected.
Similar content being viewed by others
References
Brown RD Jr, Juras H (1979) Hydrolysis of cellulose: Mechanism of enzymatic and acid catalysis. Adv Chem Ser 181, (Amer Chem Soc Washington, DC)
Cole A (1965) Cellular radiation biology. Williams and Wilkins, Baltimore, pp 267–271
Dekker RFH (1983) Bioconversion of hemicellulose: Aspects of hemicellulase production byTrichoderma reesei QM 9414 and enzymatic saccharification of hemicellulose. Biotechnol Bioeng 25:1127–1146
Elkind MM (1977) The initial part of the survival curve. Implications for low-dose, low-dose-rate radiation responses. Radiat Res 71:9–23
Erikson KE, Pettersson B (1975) Extracellular enzyme system utilized by the fungusSporotrichum pulverulentum chrysosporium lignorum) for the breakdown of cellulase. I. Separation, purification and physico-chemical characterization of five endo-1,4-β-glucanase. Eur J Biochem 51:193–199
Khare S, Jayarumar A, Trivedi A, Kesavam RC, Prasad R (1982) Radiation effects on membrane 1. Cellular permiability and cell survival. Radiat Res 90:233–243
Kumakura M, Kaetsu I, Nishizawa K (1984) Cellulase production from immobilized growing cell composites prepared by radiation polymerization. Biotechnol Bioeng 26:17–21
Kumakura M, Tamada M, Kaetsu I (1984) Properties ofTrichoderma reesei cells immobilized by the irradiation technique. Enzyme Microbiol Technol 6:411–414
Kumakura M, Kaetsu I (1984) Enzymatic hydrolysis of chaff using immobilized glowing cells. Agric Wastes 11:259–267
Mandels M, Sternberg D (1976) Recent advances in cellulase technology. J Ferment Technol 54:267–286
Myers DK (1970) Some aspects of radiation effects on cell membranes. Adv Biol Med Phys 13:219–234
Reese ET (1975) Biological transformation of wood by microorganism. Leise W (ed) Springer, New York
Zelle MR, Hollaender A (1955) In: Hollaender A (ed) Radiation Biology Vol 11, MacGraw Hill, New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kumakura, M. Dose-Dependency of radiation on enzyme production inTrichoderma reesei . Radiat Environ Biophys 32, 41–46 (1993). https://doi.org/10.1007/BF01213129
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01213129