Summary
Albumin prepared from serum of diabetic patients inhibits the effect of insulin on rat diaphragm muscle but not on their epididymal fat pads when injected intravenously to make a foreign protein concentration of 3.8 g/100 ml in the plasma of the rat. At this concentration, albumin from normal subjects had no insulin inhibitory effect on either tissue. — These observations confirm, underin vivo conditions, that diabetic albumin is more antagonistic to insulin than albumin prepared from normal subjects.
Résumé
L'albumine préparée à partir du sérum de sujets diabétiques inhibe l'effet de lïnsulme sur le muscle du diaphragme du rat mais n'a pas d'effet sur le tissu adipeux épididymaire, quand elle est injectée par voie intraveineuse pour produire une concentration de protéine étrangère de 3.8 g/100 ml dans le plasma du rat. A cette concentration, l'albumine de sujets normaux n'a pas d'effet inhibiteur sur l'insuline pour les deux tissus. — Ces observations confirment, dans les conditionsin vivo, que l'albumine diabétique est plus antagoniste de l'insuline que l'albumine obtenue à partir de sujets normaux.
Zusammenfassung
Aus diabetischen Seren präpariertes Albumin hemmt die Insulinwirkung am Zwerchfellmuskel, aber ncht am epididymalen Fettgewebe der Ratte, wenn bei i.v. Zufuhr eine Konzentration des Fremdeiweißes von 3.8 g% im Plasma der Ratten erreicht wird. Dagegen hemmt in der gleichen Konzentration verabreichtes Albumin, das aus Normalseren gewonnen wurde, den Insulineffekt an keinem der beiden Gewebe. Diese Beobachtungen bestätigen, daß auch unterin vivo Bedingungen diabetisches Albumin die Insulinwirkung stärker hemmt als das Albumin von Normalpersonen.
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Supported by grants from the British Diabetic Association and Queen's University, Belfast.
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Kammerer, L., Vallance-Owen, J. In vivo effects of the synalbumin insulin antagonist. Diabetologia 4, 336–338 (1968). https://doi.org/10.1007/BF01211768
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DOI: https://doi.org/10.1007/BF01211768