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Calcium mobilization in liver transplantation graft with warm ischemia

  • Original Articles
  • Published:
Journal of Hepato-Biliary-Pancreatic Surgery

Abstract

The influence of warm ischemia on calcium mobilization in liver transplantation was investigated. Twenty-four porcine orthotopic liver transplantations were performed by a temporary portal arterialization technique. Swine were divided into three groups according to warm ischemia time; I (0 min,n=9), II (30 min,n=8), and III (60min,n=7). Ionized calcium was measured in arterial and hepatic venous blood, in initial perfusate, and in initial perfused blood. In group I, all the pigs survived, while in group III all succumbed. In group II, four survived and four died. Ionized calcium level in influx showed no differences, but the level in the initial perfusate in group I was significantly higher than that in group III. The level in the initial perfused blood in group I was significantly higher than levels in groups II and III. Retrospective analysis in group II showed that ionized calcium value in the initial perfused blood in the survivors was significantly higher than that in the non-survivors. A substantial amount of ionized calcium accumulated after revascularization in the graft loaded with warm ischemia, and, in group II, significantly more ionized calcium accumulated in the non-survivors.

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References

  1. Goode HF, Webster NR, Howdle PD, Leek JP, Lodge JPA, Sadek SA, Walker BE (1994) Reperfusion injury, antioxidants and hemodynamics during orthotopic liver transplantation. Hepatology 19:354–359

    PubMed  Google Scholar 

  2. Mathews WR, Guido DM, Fisher MA, Jaeschke H (1994) Lipid peroxidation as molecular mechanism of liver cell injury during reperfusion after ischemia. Free Radic Biol Med 16:763–770

    PubMed  Google Scholar 

  3. Quiroga J, Prieto J (1993) Liver cytoprotection by prostaglandins. Pharmacol Ther 58:67–92

    PubMed  Google Scholar 

  4. Kim YI, Akizuki S, Kawano K, Goto S (1994) Intrahepatic neutrophil accumulation in ischemia-reperfusion injury of pig liver. Transplant Proc 26:2392–2394

    PubMed  Google Scholar 

  5. Kurita K, Tanabe G, Aikou T, Shimazu H (1993) Ischemic liver cell damage and calcium accumulation in rats. J Hepatol 18:196–204

    PubMed  Google Scholar 

  6. Uchida M, Takemoto Y, Nagasue N, Kimoto T, Dhar DK, Nakamura T (1994) Calcium in pig livers following ischemia and reperfusion. J Hepatol 20:714–719

    PubMed  Google Scholar 

  7. Acosta D, Sorensen EMB (1983) Role of calcium in cytotoxic injury of cultured hepatocytes. Ann N Y Acad Sci 407:78–92

    PubMed  Google Scholar 

  8. Fariss MW, Olafsdottir K, Reed DJ (1984) Extracellular calcium protects isolated rat hepatocytes from injury. Biochem Biophys Res Commun 121:102–110

    PubMed  Google Scholar 

  9. Brecht M, Brecht C, De Groot H (1992) Late steady increase in cytosolic Ca2+ preceding hypoxic injury in hepatocytes. Biochem J 283:399–402

    PubMed  Google Scholar 

  10. Okuda M, Lee HC, Chance B, Kumar C (1992) Role of extracellular Ca2+ in ischemia-reperfusion injury in the isolated perfused rat liver. Circ Shock 37:209–219

    PubMed  Google Scholar 

  11. Kusterer K, Blöchle C, Konrad T, Palitzsch KD, Usadel KH (1993) Rat liver injury induced by hypoxic ischemia and reperfusin: Protective action by somatostatin and two derivatives. Regul Pept 44:251–256

    PubMed  Google Scholar 

  12. Hatanaka N, Kamiike W, Shimizu S, Miyata M, Inoue T, Tagawa K, Matsuda H (1992) Ischemic liver injury induced by calcium released from mitochondria. Transplant Proc 24:1620–1622

    PubMed  Google Scholar 

  13. Shimizu S, Kamiike W, Hatanaka N, Nishimura M, Miyata M, Inoue T, Yoshida Y, Tagawa K, Matsuda H (1994) Enzyme release from mitochondria during reoxygenation of rat liver. Transplantation 57:144–148

    PubMed  Google Scholar 

  14. Tamura M, Nakajima Y, Kimura J, Omura T, Ito K, Nishikawa M, Takahashi M, Nishibe M, Aoki S, Kusumoto K, Isai H, Uchino J (1994) Experimental study on temporary portal vein arterialization in porcine liver transplantation. Transplant Proc 26:2199–2200

    PubMed  Google Scholar 

  15. Sheil AGR, Halliday JP, Drummond JM, Bookallil MJ, Gaudry PL, Yezerski SD (1972) A modified technique for orthotopic liver transplantation. Arch Surg 104:720–724

    PubMed  Google Scholar 

  16. The Enzyme Commission of the German Society for Clinical Chemistry (1972) Recommendations of the German Society for Clinical Chemistry. Z Klin Chem Klin Biochem 10:281–291

    Google Scholar 

  17. Watazu Y, Uji Y, Sugiuchi H, Okabe H, Murao S (1990) New automated measurement of mitochondrial aspartate aminotransferase with use of protease 401. Clin Chem 36:687–689

    PubMed  Google Scholar 

  18. Thomas CE, Reed DJ (1989) Current status of calcium in hepatocellular injury. Hepatology 10:375–384

    PubMed  Google Scholar 

  19. Carafoli E (1987) Intracellular calcium homeostasis. Annu Rev Biochem 56:395–433

    PubMed  Google Scholar 

  20. Schanne FAX, Kane AB, Young EE, Farber JL (1979) Calcium dependence of toxic cell death: A final common pathway. Science 206:700–702

    PubMed  Google Scholar 

  21. Nicotera P, Harzell P, Davis G, Orrenius S (1986) The formation of plasma membrane blebs in hepatocytes exposed to agents that increase cytosolic Ca2+ is mediated by the activation of a nonlysosomal proteolytic system. FEBS Lett 209:139–144

    PubMed  Google Scholar 

  22. Scherer NM, Deamer DW (1986) Oxidative stress impairs the function of sarcoplasmic reticulum by oxidation of sulfhydryl groups in the Ca2+-ATPase. Arch Biochem Biophys 246:589–601

    PubMed  Google Scholar 

  23. Inoue T, Yoshida Y, Nishimura M, Kurosawa K, Tagawa K (1993) Ca2+-induced, phospholipase-independent injury during reoxygenation of anoxic mitochondria. Biochim Biophys Acta 1140:313–320

    PubMed  Google Scholar 

  24. Brecht M, Brecht C, de Groot H (1993) Improvement of the energy status of hypoxic hepatocytes by calcium channel blockers. Res Commun Chem Pathol Pharmacol 82:185–198

    PubMed  Google Scholar 

  25. Piratvisuth T, Dunne JB, Williams R, Tredger JM (1995) Amlodipine improves hepatic hemodynamic and metabolic function in the isolated perfused rat liver after sequential cold and warm ischemia. Transplantation 60:23–28

    PubMed  Google Scholar 

  26. Uchida M, Takemoto Y, Nagasue N, Dhar DK, Kohno H, Nakamura T (1994) Effect of verapamil on hepatic reperfusion injury after prolonged ischemia in pigs. J Hepatol 21:217–223

    PubMed  Google Scholar 

  27. Hamada Y, Karjalainen A, Setchell BA, Millard JE, Bygrave FL (1992) Concomitant stimulation by vasopressin of biliary and perfusate calcium fluxes in the perfused rat liver. Biochem J 281:387–392

    PubMed  Google Scholar 

  28. Furuyashiki S, Sumimoto K, Oku J, Kimura A, Fukuda Y, Dohi K, Kawasaki T (1994) The significance of bile secretion after the transplantation of long-preserved livers in the rat. Jpn J Surg 24:59–62

    Google Scholar 

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Authors and Affiliations

  1. First Department of Surgery, Hokkaido University School of Medicine, N-15 W-7, Kita-ku, 060, Sapporo, Japan

    Hideya Isai, Jun Kimura, Yasuaki Nakajima & Junichi Uchino

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  1. Hideya Isai
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  2. Jun Kimura
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  3. Yasuaki Nakajima
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  4. Junichi Uchino
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Isai, H., Kimura, J., Nakajima, Y. et al. Calcium mobilization in liver transplantation graft with warm ischemia. J Hep Bil Pancr Surg 4, 95–102 (1997). https://doi.org/10.1007/BF01211349

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  • DOI: https://doi.org/10.1007/BF01211349

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