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Radiation and Environmental Biophysics

, Volume 29, Issue 1, pp 19–30 | Cite as

Changes in nucleoid viscosity following X-irradiation of rat thymic and splenic cells in vitro

  • K. Tempel
Article

Summary

Unscheduled DNA synthesis (UDS) suggested a higher DNA repair capacity of X-irradiated rat thymic (T) cells when compared to splenic (S) cells (Tempel 1980). In the present investigations, damage and repair of DNA supercoiling was measured in T- and S-cells following X-irradiation in vitro by using the nucleoid sedimentation technique and a simplified low-shearing viscometric test. - X-irradiation resulted in a dose (0.6–19.2 Gy) - dependent reduction in sedimentation and viscosity of nucleoids. Within a post-irradiation period of 30–45 min after a challenge dose of 19.2 Gy, DNA repair was accompanied by an increase in nucleoid sedimentation and viscosity in T-cells by about 60 and 300, in S-cells by almost 40 and 100%, resp. The increase in nucleoid viscosity within a 30 min repair period could be reduced in a concentration-dependent manner by DNA polymerase - inhibitors and proteinase K. - The higher DNA repair capacity of T-cells as reflected by UDS is confirmed therefore by the nucleoid characteristics. Apart from this suggestion, measuring nucleoid viscosity may be considered as a sensitive, simple and rapid device to detect radiation-induced DNA supercoiling phenomena.

Keywords

Sedimentation Technique Repair Capacity Challenge Dose Splenic Cell Dependent Reduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • K. Tempel
    • 1
  1. 1.Arbeitsgruppe Radiologie im Institut für PharmakologieToxikologie und Pharmazie der Tierärztlichen Fakultät der Universität MünchenMünchen 22Federal Republic of Germany

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